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Literature summary for 3.2.1.37 extracted from
- Purification and biochemical properties of a thermostable xylose-tolerant beta-D-xylosidase from Scytalidium thermophilum (2004), J. Ind. Microbiol. Biotechnol., 31, 170-176 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | beta-D-xylosidase activity is strongly stimulated and protected against heat inactivation by calcium ions | Mycothermus thermophilus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, 100% inhibition | Mycothermus thermophilus | |
| EDTA | 1 mM, 20% inhibition | Mycothermus thermophilus | |
| Hg2+ | 1 mM, 91% inhibition | Mycothermus thermophilus | |
| additional information | not inhibitory: D-xylose up to 200 mM, Mg2+, Al3+, Co2+, Mn2+, Cu2+, K+, Ba2+, Fe2+ or Zn2+ at 1.0 mM, 2-mercaptoethanol | Mycothermus thermophilus | |
| Zn2+ | 1 mM, 22% inhibition | Mycothermus thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 60°C | Mycothermus thermophilus | |
| 1.3 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 5.0, 60°C | Mycothermus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
gel filtration | Mycothermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycothermus thermophilus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | carbohydrate content 12% | Mycothermus thermophilus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Mycothermus thermophilus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 65 | - |
substrate 4-nitrophenyl beta-D-xylopyranoside, pH 5.0, 60°C | Mycothermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Mycothermus thermophilus | 4-nitrophenol + D-glucose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Mycothermus thermophilus | 4-nitrophenol + D-xylose | - |
? | |
| xylobiose + H2O | - |
Mycothermus thermophilus | 2 D-xylose | - |
? | |
| xylotetraose + 3 H2O | - |
Mycothermus thermophilus | 4 D-xylose | - |
? | |
| xylotriose + 2 H2O | - |
Mycothermus thermophilus | 3 D-xylose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 45000, SDS-PAGE | Mycothermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Mycothermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
1 h, absence of substrate, stable | Mycothermus thermophilus |
| 65 | - |
half-lives are 11 and 30 min in the absence or presence of calcium, respectively | Mycothermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Mycothermus thermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | - |
Mycothermus thermophilus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Mycothermus thermophilus | expression is induced by growth on xylan | up |
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