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Literature summary for 3.2.1.37 extracted from

  • Dodd, D.; Kocherginskaya, S.A.; Spies, M.A.; Beery, K.E.; Abbas, C.A.; Mackie, R.I.; Cann, I.K.
    Biochemical analysis of a beta-D-xylosidase and a bifunctional xylanase-ferulic acid esterase from a xylanolytic gene cluster in Prevotella ruminicola 23 (2009), J. Bacteriol., 191, 3328-3338.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.37

Cloned(Commentary)

Cloned (Comment) Organism
ecxpression in Escherichia coli Prevotella ruminicola

Protein Variants

Protein Variants Comment Organism
D269A no catalytic activity Prevotella ruminicola
D269N no catalytic activity Prevotella ruminicola
E594Q activity similar to wild-type Prevotella ruminicola
E616A about 5% of wild-type activity Prevotella ruminicola
R149L no catalytic activity Prevotella ruminicola
Y237F less than 10% of wild-type activity Prevotella ruminicola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 5.0, 37°C Prevotella ruminicola
2
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E594Q, pH 5.0, 37°C Prevotella ruminicola
3.9
-
 4-nitrophenyl-beta-D-xylopyranoside mutant Y237F, pH 5.0, 37°C Prevotella ruminicola
18
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E616A, pH 5.0, 37°C Prevotella ruminicola

Organism

Organism UniProt Comment Textmining
Prevotella ruminicola D5EY15 isoform Xyl3A
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O poor substrate Prevotella ruminicola 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Prevotella ruminicola 4-nitrophenol + beta-D-xylose
-
 ?
additional information no substrate: 4-nitrophenly-beta-D-glucopyranoside. When incubated in combination with bifunctional xylanase-ferulic acid esterase Xyn10D-Fae1A, Xyl3A improves the release of xylose monomers from a hemicellulosic xylan substrate, suggesting that these two enzymes function synergistically to depolymerize xylan Prevotella ruminicola ?
-
 ?
xylobiose + H2O
-
 Prevotella ruminicola 2 D-xylose
-
 ?
xylohexaose + H2O sole products released are xylose monomers Prevotella ruminicola 6 D-xylose
-
 ?
xylopentaose + H2O sole products released are xylose monomers Prevotella ruminicola 5 D-xylose
-
 ?
xylotetraose + 3 H2O
-
 Prevotella ruminicola 4 D-xylose
-
 ?
xylotriose + H2O
-
 Prevotella ruminicola 3 D-xylose
-
 ?

Synonyms

Synonyms Comment Organism
Xyl3A
-
 Prevotella ruminicola

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6
-
 4-nitrophenyl-beta-D-xylopyranoside mutant Y237F, pH 5.0, 37°C Prevotella ruminicola
3.3
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E616A, pH 5.0, 37°C Prevotella ruminicola
9.7
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 5.0, 37°C Prevotella ruminicola
10
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E594Q, pH 5.0, 37°C Prevotella ruminicola

General Information

General Information Comment Organism
physiological function when incubated in combination with bifunctional xylanase-ferulic acid esterase Xyn10D-Fae1A, Xyl3A improves the release of xylose monomers from a hemicellulosic xylan substrate, suggesting that these two enzymes function synergistically to depolymerize xylan Prevotella ruminicola

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.2
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E616A, pH 5.0, 37°C Prevotella ruminicola
0.41
-
 4-nitrophenyl-beta-D-xylopyranoside mutant Y237F, pH 5.0, 37°C Prevotella ruminicola
5
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E594Q, pH 5.0, 37°C Prevotella ruminicola
5.4
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 5.0, 37°C Prevotella ruminicola