Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-epoxypropyl beta-D-xylobioside | irreversible inactivation | Thermothelomyces heterothallicus | |
additional information | not inhibitory: alkyl epoxides derived from D-xylose | Thermothelomyces heterothallicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
p-nitrophenyl-beta-D-xylopyranoside | pH 7.0, 50°C | Thermothelomyces heterothallicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Thermothelomyces heterothallicus | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SDS-PAGE | Thermothelomyces heterothallicus |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | 10% v/v, 30% decrease in activity | Thermothelomyces heterothallicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermothelomyces heterothallicus | - |
strain ATCC 34628 | - |
Purification (Comment) | Organism |
---|---|
- |
Thermothelomyces heterothallicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-xylose | - |
Thermothelomyces heterothallicus | xylobiose + H2O | - |
r | |
additional information | enzyme cleaves beta-1,4-linked xylo-oligosaccharides with chain length from 2 to 6, releasing xylose from the non-reducing end. When using high donor concentration, the enzyme catalyses the condensation reaction. No substrate: xylan | Thermothelomyces heterothallicus | ? | - |
? | |
p-nitrophenyl-beta-D-xylopyranoside + H2O | - |
Thermothelomyces heterothallicus | p-nitrophenol + beta-D-xylopyranose | - |
r | |
xylobiose + H2O | - |
Thermothelomyces heterothallicus | 2 beta-D-xylose | - |
r | |
xylohexaose + H2O | 80% of the activity with xylobiose | Thermothelomyces heterothallicus | beta-D-xylose | - |
r | |
xylopentaose + H2O | 83% of the activity with xylobiose | Thermothelomyces heterothallicus | beta-D-xylose | - |
r | |
xylotetraose + H2O | 86% of the activity with xylobiose | Thermothelomyces heterothallicus | xylotriose + beta-D-xylose | - |
r | |
xylotriose + 2 H2O | 92% of the activity with xylobiose | Thermothelomyces heterothallicus | 3 D-xylose | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Thermothelomyces heterothallicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Thermothelomyces heterothallicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable up to | Thermothelomyces heterothallicus |
60 | - |
1 h, 58% residual activity | Thermothelomyces heterothallicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermothelomyces heterothallicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Thermothelomyces heterothallicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermothelomyces heterothallicus | isoelectric focusing | - |
4.2 |