Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Enterobacter cloacae |
Crystallization (Comment) | Organism |
---|---|
crystal structures are determined in the ligand-free form and bound to the inhibitor validoxylamine A. Multiple salt bridges are identified that potentially facilitate closure of a hood over the substrate-binding site | Enterobacter cloacae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
validoxylamine A | competitive inhibition | Enterobacter cloacae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.47 | - |
alpha,alpha-trehalose | 55°C, pH 5.0 | Enterobacter cloacae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Enterobacter cloacae | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
SDS-PAGE | Enterobacter cloacae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + H2O | Enterobacter cloacae | - |
beta-D-glucose + alpha-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cloacae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Enterobacter cloacae |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + H2O | - |
Enterobacter cloacae | beta-D-glucose + alpha-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Px_EclTre | - |
Enterobacter cloacae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Enterobacter cloacae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
104.25 | - |
alpha,alpha-trehalose | 55°C, pH 5.0 | Enterobacter cloacae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Enterobacter cloacae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000019 | - |
validoxylamine A | 55°C, pH 5.0 | Enterobacter cloacae |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000075 | - |
55°C, pH 5.0 | Enterobacter cloacae | validoxylamine A |