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Literature summary for 3.2.1.28 extracted from
- Identification of an extracellular acid trehalase and its gene involved in fungal pathogenesis of Metarhizium anisopliae (2006), J. Biochem., 140, 319-327.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| trehazolin | - |
Metarhizium anisopliae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.3 | - |
trehalose | in 20 mM MES buffer, pH 5.5, at 30°C | Metarhizium anisopliae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 114300 | - |
calculated from amino acid sequence | Metarhizium anisopliae |
| 130000 | - |
SDS-PAGE, deglycosylated enzyme | Metarhizium anisopliae |
| 170000 | - |
SDS-PAGE | Metarhizium anisopliae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Metarhizium anisopliae | A9XE63 | fragment; subspecies Metarhizium anisopliae acridum, strain CQMa102 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 23.5% carbohydrate content | Metarhizium anisopliae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| High Q Sepharose column chromatography, 25Q Sepharose column chromatography, and Sephacryl-200s HR gel filtration | Metarhizium anisopliae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.224 | - |
culture filtrate, in 20 mM MES buffer, pH 5.5, at 30°C | Metarhizium anisopliae |
| 120 | - |
after 98.04fold purification, in 20 mM MES buffer, pH 5.5, at 30°C | Metarhizium anisopliae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | does not hydrolyze cellobiose, maltose, sucrose, lactose, p-nitrophenyl galactopyranoside, p-nitrophenyl alpha-glucopyranoside, or p-nitrophenyl beta-glucopyranoside | Metarhizium anisopliae | ? | - |
? | |
| trehalose + H2O | highly specific for trehalose | Metarhizium anisopliae | D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acid trehalase | - |
Metarhizium anisopliae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Metarhizium anisopliae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | - |
Metarhizium anisopliae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
activity decreases rapidly at 55°C or above | Metarhizium anisopliae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Metarhizium anisopliae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | - |
Metarhizium anisopliae |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Metarhizium anisopliae | isoelectric focusing | - |
4.7 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.000013 | - |
in 20 mM MES buffer, pH 5.5, at 30°C | Metarhizium anisopliae | trehazolin | |
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