Cloned (Comment) | Organism |
---|---|
gene AciHBGase II, DNA and amino acid sequence determination and analysis, recombinant expression of the C-terminally His-tagged secretory enzyme in Pichia pastoris strain GS115 | Apis cerana indica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Apis cerana indica | |
4.2 | - |
4-nitrophenyl alpha-D-glucoside | recombinant enzyme, pH 5.5, 37°C | Apis cerana indica | |
15.3 | - |
sucrose | recombinant enzyme, pH 5.5, 37°C | Apis cerana indica | |
22 | - |
soluble starch | recombinant enzyme, pH 5.5, 37°C | Apis cerana indica | |
22.3 | - |
isomaltose | recombinant enzyme, pH 5.5, 37°C | Apis cerana indica | |
25 | - |
maltotetraose | recombinant enzyme, pH 5.5, 37°C | Apis cerana indica | |
26.7 | - |
maltotriose | recombinant enzyme, pH 5.5, 37°C | Apis cerana indica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretory enzyme | Apis cerana indica | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apis cerana indica | J9SJ08 | gene AciHBGase II | - |
Purification (Comment) | Organism |
---|---|
recombinant extracellular His-tagged enzyme 1.45fold from Pichia pastoris strain GS115 culture supernatant by ultrafiltration, nickel affinity chromatography to homogeneity | Apis cerana indica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | - |
Apis cerana indica | - |
additional information | highest transcript expression level of the isozyme is found in larvae and pupae, with lower levels in the eggs, no activity in foragers, comparison of expression patterns to isozyme I and III and to isozymes from Apis mellifera, overview | Apis cerana indica | - |
pupa | - |
Apis cerana indica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
purified recombinant His-tagged enzyme, pH 5.5, 37°C, substrate 4-nitrophenyl alpha-D-glucoside | Apis cerana indica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucoside + H2O | low activity | Apis cerana indica | 4-nitrophenol + D-glucose | - |
? | |
isomaltose + H2O | low activity | Apis cerana indica | ? | - |
? | |
maltose + H2O | low activity | Apis cerana indica | ? | - |
? | |
maltotetraose + H2O | low activity | Apis cerana indica | ? | - |
? | |
maltotriose + H2O | low activity | Apis cerana indica | ? | - |
? | |
additional information | the enzyme shows a wide substrate specificity cleaving alpha-(1,1)-, alpha-(1,2)-, alpha-(1,4)- and alpha-(1,6)-glucosidic linkages, and rapidly hydrolyzing 4-nitrophenyl alpha-D-glucoside alpha-(1,6) and sucrose alpha-(1,2) linkages. Recombinant and native display positive cooperativity to sucrose, turanose alpha-(1,3), kojibiose alpha-(1,2) and soluble starch alpha-(1,4), the recombinant His-tagged enzyme hydrolyzes isomaltose and soluble starch but with different Km values | Apis cerana indica | ? | - |
? | |
soluble starch + H2O | low activity | Apis cerana indica | ? | - |
? | |
sucrose + H2O | highly preferred substrate | Apis cerana indica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-glucosidase II | - |
Apis cerana indica |
HBGase II | - |
Apis cerana indica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant His-tagged enzyme | Apis cerana indica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 55 | recombinant His-tagged enzyme, over 80% of maximal activity in this range | Apis cerana indica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 65 | purified recombinant His-tagged enzyme, over 80% activity remaining | Apis cerana indica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.89 | - |
4-nitrophenyl alpha-D-glucoside | recombinant enzyme, pH 5.5, 37°C | Apis cerana indica | |
2.84 | - |
maltotetraose | recombinant enzyme, pH 5.5, 37°C | Apis cerana indica | |
4.6 | - |
soluble starch | recombinant enzyme, pH 5.5, 37°C | Apis cerana indica | |
5.2 | - |
isomaltose | recombinant enzyme, pH 5.5, 37°C | Apis cerana indica | |
26.6 | - |
maltotriose | recombinant enzyme, pH 5.5, 37°C | Apis cerana indica | |
845 | - |
sucrose | recombinant enzyme, pH 5.5, 37°C | Apis cerana indica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
recombinant His-tagged enzyme | Apis cerana indica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 5 | recombinant His-tagged enzyme, over 80% of maximal activity in this range | Apis cerana indica |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | purified recombinant His-tagged enzyme, over 80% activity remaining | Apis cerana indica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
maltotetraose | recombinant enzyme, pH 5.5, 37°C | Apis cerana indica | |
0.21 | - |
soluble starch | recombinant enzyme, pH 5.5, 37°C | Apis cerana indica | |
0.23 | - |
isomaltose | recombinant enzyme, pH 5.5, 37°C | Apis cerana indica | |
0.45 | - |
4-nitrophenyl alpha-D-glucoside | recombinant enzyme, pH 5.5, 37°C | Apis cerana indica | |
1 | - |
maltotriose | recombinant enzyme, pH 5.5, 37°C | Apis cerana indica | |
55.4 | - |
sucrose | recombinant enzyme, pH 5.5, 37°C | Apis cerana indica |