Application | Comment | Organism |
---|---|---|
industry | amylases are important industrial enzymes used in starch liquefaction to produce glucose, fructose and maltose and also in brewing, baking, textile, paper, detergent and sugar industries | Abrus precatorius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km and Vmax values are 79.37mg/ml and 5.13 U/ml, respectively, at 50°C, pH 6.0, Michaelis-Menten kinetics | Abrus precatorius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Abrus precatorius | - |
harvested in Ngaoundere, Adamawa, region in Northern Cameroon | - |
Purification (Comment) | Organism |
---|---|
native enzyme enriched fraction from powdered stems of Abrus precatorius using a three-phase partitioning method and a Doehlert design with 3 variables (ratio of crude extract/t-butanol, the ammonium sulfate saturation and pH). Data fitting in a second-order polynomial model and parameters optimization to enrich beta-amylase. Optimal conditions are: a ratio of crude extract/tert-butanol of 0.87 v/v, saturation in ammonium sulfate of 49.46% w/v, and a pH of 5.2. An activity recovery of 156.2% and a purification factor of 10.17 are found. Next steps are dialysis and lyophilisation | Abrus precatorius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | - |
Abrus precatorius | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.79 | - |
crude enzyme extract, pH 6.0, 50°C | Abrus precatorius |
19.99 | - |
TPP-interfacial precipitate enzyme after dialysis and lyophilisation, pH 6.0, 50°C | Abrus precatorius |
24.59 | - |
TPP-interfacial precipitate enzyme after dialysis, pH 6.0, 50°C | Abrus precatorius |
38.24 | - |
TPP-interfacial precipitate enzyme, pH 6.0, 50°C | Abrus precatorius |
Subunits | Comment | Organism |
---|---|---|
? | x * 60100, SDS-PAGE | Abrus precatorius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
native enzyme | Abrus precatorius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
purified native enzyme, pH 6.0-6.5, stable up to | Abrus precatorius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | native enzyme | Abrus precatorius |