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Literature summary for 3.2.1.191 extracted from

  • Cui, C.; Kim, D.; Jung, S.; Kim, S.; Im, W.
    Enhanced production of gypenoside LXXV using a novel ginsenoside-transforming beta-glucosidase from ginseng-cultivating soil bacteria and its anti-cancer property (2017), Molecules, 22, 844-858 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.191

Application

Application Comment Organism
synthesis the enzyme is applied for the production of gypenoside LXXV from gypenoside XVII at the gram-scale. The pure GypLXXV can be used for the study of anti-cancer effects against three kinds of cancer cells in vitro Microbacterium sp.

Cloned(Commentary)

Cloned (Comment) Organism
BglG167b cloning and overexpression in Escherichia coli as GST-tagged enzyme, phylogenetic analysis of BglG167b Microbacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Microbacterium sp.
Co2+
-
 Microbacterium sp.
Cu2+
-
 Microbacterium sp.
EDTA
-
 Microbacterium sp.
SDS
-
 Microbacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme activity is not affected by Na+, K+, Mg2+, and Mn2+ Microbacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ginsenoside 20(S)-Rg3 + H2O Microbacterium sp.
-
 ginsenoside 20(S)-Rh2 + D-glucopyranose
-
 ?
ginsenoside F2 + H2O Microbacterium sp.
-
 ginsenoside C-K + D-glucopyranose
-
 ?
ginsenoside F2 + H2O Microbacterium sp. Gsoil 167
-
 ginsenoside C-K + D-glucopyranose
-
 ?
ginsenoside Rb2 + H2O Microbacterium sp.
-
 ginsenoside C-O + D-glucopyranose
-
 ?
ginsenoside Rb2 + H2O Microbacterium sp. Gsoil 167
-
 ginsenoside C-O + D-glucopyranose
-
 ?
gypenoside XVII + H2O Microbacterium sp.
-
 gypenoside LXXV + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Microbacterium sp. L0EKQ7 from ginseng-cultivating soil
-
Microbacterium sp. Gsoil 167 L0EKQ7 from ginseng-cultivating soil
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography Microbacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-glucopyranoside + H2O low activity Microbacterium sp. 2-nitrophenol + beta-D-glucose
-
 ?
2-nitrophenyl beta-D-glucopyranoside + H2O low activity Microbacterium sp. Gsoil 167 2-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl alpha-D-glucopyranoside + H2O very low activity Microbacterium sp. 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Microbacterium sp. 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Microbacterium sp. Gsoil 167 4-nitrophenol + beta-D-glucose
-
 ?
ginsenoside 20(S)-Rg3 + H2O
-
 Microbacterium sp. ginsenoside 20(S)-Rh2 + D-glucopyranose
-
 ?
ginsenoside F2 + H2O
-
 Microbacterium sp. ginsenoside C-K + D-glucopyranose
-
 ?
ginsenoside F2 + H2O
-
 Microbacterium sp. Gsoil 167 ginsenoside C-K + D-glucopyranose
-
 ?
ginsenoside Rb2 + H2O
-
 Microbacterium sp. ginsenoside C-O + D-glucopyranose
-
 ?
ginsenoside Rb2 + H2O
-
 Microbacterium sp. Gsoil 167 ginsenoside C-O + D-glucopyranose
-
 ?
gypenoside XVII + H2O
-
 Microbacterium sp. gypenoside LXXV + D-glucose
-
 ?
additional information enzyme can hydrolyze various ginsenosides, i.e. Rb1, Rb2, Rc, Rd, Rg3(S), F2, Re, Rg1, GypXVII, through various cleavage pathways, substrate specificity, overview. BglG167b cannot hydrolyze the attached sugars at C-6, but glucose moieties at C-3 Microbacterium sp. ?
-
 ?
additional information enzyme can hydrolyze various ginsenosides, i.e. Rb1, Rb2, Rc, Rd, Rg3(S), F2, Re, Rg1, GypXVII, through various cleavage pathways, substrate specificity, overview. BglG167b cannot hydrolyze the attached sugars at C-6, but glucose moieties at C-3 Microbacterium sp. Gsoil 167 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 90300, about, sequence calculation, x * 116000, about, recombinant GST-tagged enzyme BglG167b, sequence calculation Microbacterium sp.

Synonyms

Synonyms Comment Organism
BglG167b
-
 Microbacterium sp.
ginsenoside-transforming beta-glucosidase
-
 Microbacterium sp.
More cf. EC 3.2.1.194 Microbacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 recombinant enzyme Microbacterium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45 at 30°C and 37°C, the recombinant enzyme has 75.0% and 89.8% of the maximal activity at 45°C Microbacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5 30 purified recombinant enzyme, 2 h, over 90% activity remaining Microbacterium sp.
40
-
 purified recombinant enzyme, 2 h, over 80% activity remaining Microbacterium sp.
45
-
 purified recombinant enzyme, 2 h, no activity remaining, inactivation above 45°C Microbacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 recombinant enzyme Microbacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 over 50% of maximal activity within this range, recombinant enzyme Microbacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 purified recombinant enzyme, 1.2% activity remaining Microbacterium sp.
6 9 purified recombinant enzyme, over 80% activity remaining Microbacterium sp.
8.5 9 purified recombinant enzyme, completely stable at Microbacterium sp.
10
-
 purified recombinant enzyme, 40% activity remaining Microbacterium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Microbacterium sp. sequence calculation
-
 4.56

General Information

General Information Comment Organism
evolution the enzyme shows homology with glycosyl hydrolases from family 3, GH3 Microbacterium sp.
metabolism transformation pathways of ginsenosides by enzyme BglG167b, overview Microbacterium sp.
additional information enhanced production of gypenoside LXXV using a ginsenoside-transforming beta-glucosidase from ginseng-cultivating soil bacteria and its anti-cancer property. Anti-cancer effects of GypLXXV, Rg3(S), GypXVII and Rb1 on cell viability of HeLa cells, B16 cells, and MDA-MB23 cells Microbacterium sp.