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Literature summary for 3.2.1.191 extracted from

  • An, D.S.; Cui, C.H.; Lee, H.G.; Wang, L.; Kim, S.C.; Lee, S.T.; Jin, F.; Yu, H.; Chin, Y.W.; Lee, H.K.; Im, W.T.; Kim, S.G.
    Identification and characterization of a novel Terrabacter ginsenosidimutans sp. nov. beta-glucosidase that transforms ginsenoside Rb1 into the rare gypenosides XVII and LXXV (2010), Appl. Environ. Microbiol., 76, 5827-5836.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.191

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Terrabacter ginsenosidimutans

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 10 mM, complete inhibition Terrabacter ginsenosidimutans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 ginsenoside Rb1 pH 7.0, 37°C Terrabacter ginsenosidimutans
4.2
-
 4-nitrophenyl beta-D-glucopyranoside pH 7.0, 37°C Terrabacter ginsenosidimutans

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not require Mg2+, Mn2+, Co2+, Zn2+, Ca2+, or Cu2+ for activity Terrabacter ginsenosidimutans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 3 * 70000, SDS-PAGE Terrabacter ginsenosidimutans
71140
-
 3 * 71140, SDS-PAGE Terrabacter ginsenosidimutans
193000
-
 gel filtration Terrabacter ginsenosidimutans

Organism

Organism UniProt Comment Textmining
Terrabacter ginsenosidimutans D2KAT1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Terrabacter ginsenosidimutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Terrabacter ginsenosidimutans 2-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Terrabacter ginsenosidimutans 4-nitrophenol + D-glucopyranose
-
 ?
ginsenoside Rb1 + H2O hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond Terrabacter ginsenosidimutans gypenoside XVII + D-glucopyranose
-
 ?
gypenoside XVII + H2O hydrolysis of 3-O-beta-D-glucopyranosyl bond Terrabacter ginsenosidimutans gypenoside LXXV + D-glucopyranose
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 70000, SDS-PAGE Terrabacter ginsenosidimutans
trimer 3 * 71140, SDS-PAGE Terrabacter ginsenosidimutans

Synonyms

Synonyms Comment Organism
bgpA
-
 Terrabacter ginsenosidimutans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Terrabacter ginsenosidimutans
45
-
-
 Terrabacter ginsenosidimutans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 the enzyme is stable at temperatures lower than 37°C Terrabacter ginsenosidimutans
45
-
 about 32% of the activity is lost after incubation at 45°C for 30 min Terrabacter ginsenosidimutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 in both sodium phosphate and McIlvaine buffers Terrabacter ginsenosidimutans

pH Range

pH Minimum pH Maximum Comment Organism
6 8 the enzyme retains over 82% of its optimal activity between pH values 6.0 and 8.0. It exhibits residual activity at pH 5.0, 9.0, and 10.0, and it shows no activity at pH 4.0 Terrabacter ginsenosidimutans