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Literature summary for 3.2.1.176 extracted from

  • Ranaei Siadat, S.; Mollasalehi, H.; Heydarzadeh, N.
    Substrate affinity and catalytic efficiency are improved by decreasing glycosylation sites in Trichoderma reesei cellobiohydrolase I expressed in Pichia pastoris (2016), Biotechnol. Lett., 38, 483-488 .
    View publication on PubMed
Search for more literature for 3.2.1.176

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Pichia Pink strain2 Trichoderma reesei

Protein Variants

Protein Variants Comment Organism
N45A the mutant shows 114% of wild type activity Trichoderma reesei
N45A/N64A the mutant shows 143% of wild type activity Trichoderma reesei
N64A the mutant shows 106% of wild type activity Trichoderma reesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellulose + H2O Trichoderma reesei
-
 cellobiose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trichoderma reesei
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
 Trichoderma reesei ?
-
 ?
cellulose + H2O
-
 Trichoderma reesei cellobiose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 100000, glycosylated recombinant enzyme, SDS-PAGE Trichoderma reesei
? x * 65000, deglycosylated recombinant enzyme, SDS-PAGE Trichoderma reesei

Synonyms

Synonyms Comment Organism
CBH I
-
 Trichoderma reesei
Cel7A
-
 Trichoderma reesei
cellobiohydrolase I
-
 Trichoderma reesei
exoglucanase
-
 Trichoderma reesei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Trichoderma reesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Trichoderma reesei