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Information on EC 3.2.1.176 - cellulose 1,4-beta-cellobiosidase (reducing end)
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3.2.1.176 cellulose 1,4-beta-cellobiosidase (reducing end)IUBMB Comments
Some exocellulases, most of which belong to the glycoside hydrolase family 48 (GH48, formerly known as cellulase family L), act at the reducing ends of cellulose and similar substrates. The CelS enzyme from Clostridium thermocellum is the most abundant subunit of the cellulosome formed by the organism. It liberates cellobiose units from the reducing end by hydrolysis of the glycosidic bond, employing an inverting reaction mechanism . Different from EC 3.2.1.91, which attacks cellulose from the non-reducing end.
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Word Map
- 3.2.1.176
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crew
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subsystem
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mission
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hydroponic
- cellulosome
- thermocellum
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bioregenerative
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life-support
- cellulases
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lunar
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long-duration
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inedible
- degradation
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breadboard
- avicel
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dockerin
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moon
- synthesis
- biotechnology
- biofuel production
- analysis
- paper production
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
2
+
2
=
2
+
+
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and similar substrates, releasing cellobiose from the reducing ends of the chains.
Synonyms
celss, cel48s, cbh-1, cbhi.1, cel48c, gh7 cbh, cbh7b, cellobiohydrolase cels, 1,4-beta-d-glucan cellobiohydrolase i, exoglucanase s, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
1,4-beta-D-glucan cellobiohydrolase I
1,4-beta-D-glucan-cellobiohydrolase I
CBH
CBH I
CBH1
Cbh7B
CbhA
CBHI
Cel48A
Cel48C
Cel48S
Cel7A
Cel7B
Cel7D
Cel9B
cellobiohydrolase
cellobiohydrolase CelS
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-
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cellobiohydrolase class I
cellobiohydrolase I
Cellulase SS
CelO
celS
CelSS
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-
-
-
CelY
Ct-Cel5F
endoglucanase SS
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-
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exo-beta-1,4-glucanase
exoglucanase
GH7 CBH
reducing end acting processive exocellulase
reducing end-acting CBH
THITE_62596
additional information
Cel48A
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-
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Cel7A
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cellobiohydrolase
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cellobiohydrolase I
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Cellulase SS
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celS
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additional information
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the enzyme Cel7A probably belongs to the cellobiohydrolase family 7
additional information
the enzyme Cel7D belongs to the glycoside hydrolase family 7
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and similar substrates, releasing cellobiose from the reducing ends of the chains.
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2 cellohexaose + 2 H2O = 2 cellotriose + cellotetraose + cellobiose
mechanism
2 cellohexaose + 2 H2O = 2 cellotriose + cellotetraose + cellobiose
double-displacement mechanism, transition states, formation of an glycosyl-enzyme intermediate, structure-reactivity studies, the catalytic triad is formed by Glu212, Asp214, and Glu217, the glycosylation step is rate-limiting, overview
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2 cellohexaose + 2 H2O = 2 cellotriose + cellotetraose + cellobiose
substrate binding structure analysis of Cel7D
2 cellohexaose + 2 H2O = 2 cellotriose + cellotetraose + cellobiose
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
4-beta-D-glucan cellobiohydrolase (reducing end)
Some exocellulases, most of which belong to the glycoside hydrolase family 48 (GH48, formerly known as cellulase family L), act at the reducing ends of cellulose and similar substrates. The CelS enzyme from Clostridium thermocellum is the most abundant subunit of the cellulosome formed by the organism. It liberates cellobiose units from the reducing end by hydrolysis of the glycosidic bond, employing an inverting reaction mechanism [2]. Different from EC 3.2.1.91, which attacks cellulose from the non-reducing end.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(cellobiose)n + H2O
(cellobiose)n-1 + cellobiose
-
release of cellobiose from the reducing end. The enzyme uses a single displacement mechanism resulting in inversion of the anomeric configuration
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-
?
2,4-dinitrophenyl beta-cellobioside + H2O
2,4-dinitrophenol + beta-cellobiose
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-
-
-
?
2,4-dinitrophenyl beta-glucopyranoside + H2O
2,4-dinitrophenol + beta-D-glucose
-
-
-
-
?
2,4-dinitrophenyl beta-lactoside + H2O
2,4-dinitrophenol + beta-lactose
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-
-
-
?
2-chloro-4-nitrophenyl beta-cellobioside + H2O
2-chloro-4-nitrophenol + beta-cellobiose
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-
-
-
?
2-chloro-4-nitrophenyl beta-D-lactoside + H2O
2-chloro-4-nitrophenol + D-lactose
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-
-
?
2-chloro-4-nitrophenyl beta-D-lactoside + H2O
2-chloro-4-nitrophenol + lactose
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-
-
?
2-chloro-4-nitrophenyl beta-lactoside + H2O
2-chloro-4-nitrophenol + beta-lactose
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-
-
-
?
2-chloro-4-nitrophenyl lactoside + H2O
2-chloro-4-nitrophenol + ?
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-
-
?
2-chloro-4-nitrophenyl-beta-D-cellotrioside + H2O
2-chloro-4-nitrophenol + ?
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-
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?
2-chloro-4-nitrophenyl-beta-D-lactoside + H2O
2-chloro-4-nitrophenol + D-lactose
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-
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?
4-bromophenyl beta-cellobioside + H2O
4-bromophenol + beta-cellobiose
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-
-
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?
4-bromophenyl beta-glucopyranoside + H2O
4-bromophenol + beta-D-glucose
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-
-
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?
4-methylumbelliferyl beta-cellobioside + H2O
4-methylumbelliferone + cellobiose
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-
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?
4-methylumbelliferyl beta-cellopentaoside + H2O
4-methylumbelliferone + cellopentaose
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-
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?
4-methylumbelliferyl beta-cellotetraoside + H2O
4-methylumbelliferone + cellotetraose
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-
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?
4-methylumbelliferyl beta-cellotrioside + H2O
4-methylumbelliferone + cellotriose
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-
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?
4-methylumbelliferyl beta-lactoside + H2O
4-methylumbelliferone + lactose
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?
4-methylumbelliferyl-beta-D-lactoside + H2O
4-methylumbelliferone + lactose
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?
4-nitrophenyl beta-cellobioside + H2O
4-nitrophenol + beta-cellobiose
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?
4-nitrophenyl beta-D-cellopentaoside + H2O
4-nitrophenol + beta-D-cellopentaose
best substrate
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?
4-nitrophenyl beta-D-cellotrioside + H2O
4-nitrophenol + cellobiose
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?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
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-
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?
4-nitrophenyl beta-D-lactopyranoside + H2O
4-nitrophenol + D-lactose
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?
4-nitrophenyl beta-D-lactoside
4-nitrophenol + beta-D-lactose
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-
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?
4-nitrophenyl beta-D-lactoside + H2O
4-nitrophenol + beta-D-lactose
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?
4-nitrophenyl beta-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
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?
4-nitrophenyl beta-lactoside + H2O
4-nitrophenol + beta-lactose
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?
4-trifluoromethylumbelliferyl beta-cellobioside + H2O
4-trifluoromethylumbelliferone + cellobiose
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?
4-trifluoromethylumbelliferyl beta-cellotrioside + H2O
4-trifluoromethylumbelliferone + cellotriose
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?
Avicel PH 101 + H2O
?
cellobiose production is monitored by an amperometric enzyme biosensor based on cellobiose dehydrogenase from Phanerochaete chrysosporium adsorbed onto the surface of a benzoquinone-modified carbon paste electrode
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?
bacterial cellulose + H2O
cellobiose + cellotriose
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no release of D-.glucose is observed
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?
barley straw + H2O
?
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investigation of the action of the commercial cellulase product Celluclast 1.5 (a mixture of different cellulases), derived from Trichoderma reesei on barley straw
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?
carboxymethylcellulose + H2O
glucose + cellobiose + cellutetraose
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?