Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.161 extracted from

  • Daiyasu, H.; Saino, H.; Tomoto, H.; Mizutani, M.; Sakata, K.; Toh, H.
    Computational and experimental analyses of furcatin hydrolase for substrate specificity studies of disaccharide-specific glycosidases (2008), J. Biochem., 144, 467-475.
    View publication on PubMed
Search for more literature for 3.2.1.161

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli BL21 transformed with expression plasmid pGEX Viburnum furcatum

Protein Variants

Protein Variants Comment Organism
A419V furcatin: 40%, pNP beta-primeveroside: 78% and kcat/Km (min-1 * mM-1) = 1.3, pAP beta-glucoside: 56%, pNP beta-glucoside: 127% activity compared to wild-type Viburnum furcatum
A419W furcatin: 26%, pNP beta-primeveroside: 24% and kcat/Km (min-1 * mM-1) = 0.59, pAP beta-glucoside: 43%, pNP beta-glucoside: 39% activity compared to wild-type Viburnum furcatum
E501A furcatin: 0.1% activity compared to wild-type Viburnum furcatum
E501D furcatin: 0.2% activity compared to wild-type Viburnum furcatum
E501L furcatin: 0.1% activity compared to wild-type Viburnum furcatum
E501Q furcatin: 0.1% activity compared to wild-type Viburnum furcatum
S504A furcatin: 113%, pNP beta-primeveroside: 62% and kcat/Km (min-1 * mM-1) = 0.75, pAP beta-glucoside: 87%, pNP beta-glucoside: 87% activity compared to wild-type Viburnum furcatum
S504W furcatin: 1.4%, pNP beta-primeveroside: 1.3% and kcat/Km (min-1 * mM-1) = 0.013, pAP beta-glucoside: 20%, pNP beta-glucoside: 28% activity compared to wild-type Viburnum furcatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
 p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside 37°C, pH 5.0, A419W Viburnum furcatum
1.9
-
 p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside 37°C, pH 5.0, A419V Viburnum furcatum
2 3 p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside 37°C, pH 5.0, S504W Viburnum furcatum
4
-
 p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside 37°C, pH 5.0, wild-type Viburnum furcatum
7.5
-
 p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside 37°C, pH 5.0, S504A Viburnum furcatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88000
-
 SDS-PAGE of eluted fractions Viburnum furcatum

Organism

Organism UniProt Comment Textmining
Viburnum furcatum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of supernatant with glutathion-Sepharose column after centrifugation of cell lysate Viburnum furcatum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.27
-
 wild-type enzyme with substrate furcatin, release of p-allylphenol Viburnum furcatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-allylphenyl beta-D-glucoside + H2O pAP beta-glucoside, 2 mM substrate, 37°C, 20 mM sodium citrate buffer, pH 5.0 Viburnum furcatum 4-allylphenol + beta-D-glucose
-
 ?
p-allylphenyl 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O pAP furcatin, 2 mM substrate, 37°C, 20 mM sodium citrate buffer, pH 5.0 Viburnum furcatum p-allylphenol + 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside aglycon product acts as insect deterrent ?
p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O pNP beta-primeveroside, 2 mM substrate, 37°C, 20 mM sodium citrate buffer, pH 5.0, measurement of kinetic parameters Viburnum furcatum p-nitrophenol + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
-
 ?
p-nitrophenyl beta-D-glucoside + H2O 2 mM substrate, 37°C, 20 mM sodium citrate buffer, pH 5.0 Viburnum furcatum p-nitrophenol + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
FH
-
 Viburnum furcatum
furcatin hydrolase disaccharide-specific glycosidase Viburnum furcatum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside 37°C, pH 5.0, S504W Viburnum furcatum
0.017
-
 p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside 37°C, pH 5.0, A419W Viburnum furcatum
0.042
-
 p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside 37°C, pH 5.0, A419V Viburnum furcatum
0.093
-
 p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside 37°C, pH 5.0, S504A Viburnum furcatum
0.098
-
 p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside 37°C, pH 5.0, wild-type Viburnum furcatum