Cloned (Comment) | Organism |
---|---|
48 h in Nicotiana benthamiana leaves infiltrated by Agrobacterium tumefaciens C58C1 with entry vector pDONR(TM)Zeo and destination vector pMDC32 | Cichorium intybus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | proteinaceous inhibitors of acid invertases (structurally related to fructan 1-exohydrolase) do not inhibit fructan 1-exohydrolase | Cichorium intybus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cichorium intybus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
leaf material frozen, ground, soluble proteins extracted, precipitated, dialyzed, cell-wall associated proteins in pellets incubated in 1 M CaCl, supernatant precipitated, dialysed | Cichorium intybus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Cichorium intybus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0122 | - |
of 1-FEH I with 0.0083 micomol/min/mg background activity in Nicotiana benthamiana leaves | Cichorium intybus |
0.0205 | - |
of 1-FEH IIa with 0.0083 micomol/min/mg background activity in Nicotiana benthamiana leaves | Cichorium intybus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,1,1-kestopentaose + H2O | 1.2 mM in 50 mM Na-acetate buffer, pH 5.2, 0.02% Na-azide, 60 min, 30°C | Cichorium intybus | D-fructose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-FEH IIa | - |
Cichorium intybus |
fructan 1-exohydrolase | - |
Cichorium intybus |