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Literature summary for 3.2.1.153 extracted from
- Purification and properties of a second fructan exohydrolase from the roots of Chicorium intybus (1999), Physiol. Plant., 106, 28-34.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 1 mM, partial | Cichorium intybus |  |
| Na-EDTA | 1 mM, partial | Cichorium intybus | |
| sucrose | strong | Cichorium intybus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 58 | - |
1-kestose | - |
Cichorium intybus | |
| 64 | - |
1,1-nystose | - |
Cichorium intybus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
gel filtration | Cichorium intybus |
| 64000 | - |
1 * 64000, SDS-PAGE | Cichorium intybus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cichorium intybus | - |
L. var. foliosum cv. Flash | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cichorium intybus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Cichorium intybus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,1-nystose + H2O | - |
Cichorium intybus | ? | - |
? | |
| 1-kestose + H2O | - |
Cichorium intybus | ? | - |
? | |
| inulin + H2O | - |
Cichorium intybus | fructose + ? | - |
? | |
| additional information | the enzyme hydrolyzes the beta(2-1) linkage in inulin, 1-kestose and 1,1-nystose at rates at least 5fold faster than the beta(2-6) linkages in levan oligosaccharides and levanbiose | Cichorium intybus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 64000, SDS-PAGE | Cichorium intybus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1-fructan exohydrolase | - |
Cichorium intybus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Cichorium intybus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | - |
Cichorium intybus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.9 | - |
sucrose | - |
Cichorium intybus | |
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