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Literature summary for 3.2.1.149 extracted from

  • Sakata, K.; Mizutani, M.; Ma, S.J.; Hiratake, J.
    Diglycoside-specific glycosidases (2003), Methods Enzymol., 363, 444-459.
    View publication on PubMed
Search for more literature for 3.2.1.149

Cloned(Commentary)

Cloned (Comment) Organism
-
 Camellia sinensis
expression in Escherichia coli Camellia sinensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 2-phenylethyl beta-primeveroside pH 6.0, 37°C Camellia sinensis
14.9
-
 2-phenylethyl beta-vicianoside pH 6.0, 37°C Camellia sinensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60170
-
 TOFMS analysis Camellia sinensis
60310
-
 TOFMS analysis Camellia sinensis
60480
-
 TOFMS analysis Camellia sinensis
61000
-
 1 * 61000, SDS-PAGE, TOFMS analysis Camellia sinensis

Organism

Organism UniProt Comment Textmining
Camellia sinensis Q7X9A9 var assamica
-
Camellia sinensis Q7X9A9 var sinensis cv Shuixian
-
Camellia sinensis Q7X9A9 var sinensis cv Yabukita
-

Purification (Commentary)

Purification (Comment) Organism
-
 Camellia sinensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.9
-
 measured with p-nitrophenol beta-primeveroside in 20 mM citrate buffer (pH 6.0) at 37°C Camellia sinensis
0.98
-
 measured with p-nitrophenol beta-primeveroside in 20 mM citrate buffer (pH 6.0) at 37°C Camellia sinensis
0.99
-
 measured with p-nitrophenol beta-primeveroside in 20 mM citrate buffer (pH 6.0) at 37°C Camellia sinensis
1.7
-
 purified enzyme Camellia sinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phenylethyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O pH 6.0, 37°C, 0.05% activity Camellia sinensis 2-phenylethyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranose + 2-phenylethanol
-
 ?
2-phenylethyl 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O pH 6.0, 37°C, 0.60% activity Camellia sinensis beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranose + 2-phenylethanol
-
 ?
2-phenylethyl beta-gentiobioside + H2O pH 6.0, 37°C, 0.34% activity Camellia sinensis gentiobiose + 2-phenylethanol
-
 ?
2-phenylethyl beta-primeveroside + H2O pH 6.0, 37°C Camellia sinensis primeverose + 2-phenylethanol
-
 ?
2-phenylethyl beta-primeveroside + H2O pH 6.0, 37°C, 100% activity Camellia sinensis primeverose + 2-phenylethanol
-
 ?
2-phenylethyl beta-vicianoside + H2O pH 6.0, 37°C, 2.97% activity Camellia sinensis vicianose + 2-phenylethanol
-
 ?
p-nitrophenol beta-D-glucopyranoside + H2O pH 6.0, 37°C, 0.23% activity Camellia sinensis D-glucose + p-nitrophenol
-
 ?
p-nitrophenyl beta-primeveroside + H2O pH 6.0, 37°C Camellia sinensis primeverose + p-nitrophenol
-
 ?
p-nitrophenyl beta-primeveroside + H2O pH 6.0, 37°C, 54.4% activity Camellia sinensis primeverose + p-nitrophenol
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 61000, SDS-PAGE, TOFMS analysis Camellia sinensis

Synonyms

Synonyms Comment Organism
beta-primeverosidase
-
 Camellia sinensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Camellia sinensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 estimated from the residual activities after incubation in 20 mM citrate buffer (pH 6.0) at various temperatures (30-70°C) Camellia sinensis
45
-
 estimated from the residual activities after incubation in 20 mM citrate buffer (pH 6.0) at various temperatures (30-70°C) Camellia sinensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.83
-
 2-phenylethyl beta-vicianoside pH 6.0, 37°C Camellia sinensis
43.8
-
 2-phenylethyl beta-primeveroside pH 6.0, 37°C Camellia sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Camellia sinensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 5
-
 Camellia sinensis
4 5
-
 Camellia sinensis

pI Value

Organism Comment pI Value Maximum pI Value
Camellia sinensis
-
-
 9.4
Camellia sinensis
-
-
 9.5