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Information on EC 3.2.1.149 - beta-primeverosidase
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3.2.1.149 beta-primeverosidaseIUBMB Comments
The enzyme is responsible for the formation of the alcoholic aroma in oolong and black tea. In addition to beta-primeverosides [i.e. 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranosides], it also hydrolyses 6-O-(beta-D-apiofuranosyl)-beta-D-glucopyranosides and, less rapidly, beta-vicianosides and 6-O-(alpha-L-arabinofuranosyl)-beta-D-glucopyranosides, but not beta-glucosides. Geranyl-, linaloyl-, benzyl- and p-nitrophenol glycosides are all hydrolysed.
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Word Map
- 3.2.1.149
- beta-d-galactosidase
- alpha-d-mannosidase
- beta-d-glucuronidase
- glycosidases
- alpha-d-glucosidase
- alpha-l-fucosidase
- beta-d-glucoside
- beta-d-fucosidase
- alpha-d-galactosidase
-
3.2.1.21
- beta-d-xylosidase
-
n-acetyl-beta-d-glucosaminidase
- almond
- p-nitrophenyl-beta-d-glucopyranoside
- alpha-l-arabinosidase
- gentiobiose
-
beta-d-n-acetylglucosaminidase
- emulsin
- beta-d-xylopyranoside
- n-acetyl-beta-d-galactosaminidase
- nutrition
The enzyme appears in viruses and cellular organisms
Synonyms
beta-d-glucosidase, beta-primeverosidase, beta-glucoside hydrolase, 4-methylumbelliferyl-beta-d-glucopyranoside:beta-glucosidase, arbutinase, primeverosidase, ary beta-glucosidase, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
4-methylumbelliferyl-beta-D-glucopyranoside:beta-glucosidase
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arbutinase
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ary beta-glucosidase
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beta-D-glucosidase
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beta-D-glucoside glucohydrolase
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beta-glucosidase
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beta-glucoside hydrolase
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beta-primeverosidase
p-nitrophenyl beta-glucosidase
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primeverosidase
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vicianase
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beta-primeverosidase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O = 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose + an alcohol
The enzyme is responsible for the formation of the alcoholic aroma in oolong and black tea. In addition to beta-primeverosides, i.e. 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranosides, it also hydrolyses 6-O-(beta-D-apiofuranosyl)-beta-D-glucopyranosides and, less rapidly, beta-vicianosides and 6-O-(alpha-L-arabinofuranosyl)-beta-D-glucopyranosides, but not beta-glucosides. Geranyl-, linaloyl-, benzyl- and p-nitrophenol glycosides are all hydrolysed
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside 6-O-(beta-D-xylosyl)-beta-D-glucohydrolase
The enzyme is responsible for the formation of the alcoholic aroma in oolong and black tea. In addition to beta-primeverosides [i.e. 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranosides], it also hydrolyses 6-O-(beta-D-apiofuranosyl)-beta-D-glucopyranosides and, less rapidly, beta-vicianosides and 6-O-(alpha-L-arabinofuranosyl)-beta-D-glucopyranosides, but not beta-glucosides. Geranyl-, linaloyl-, benzyl- and p-nitrophenol glycosides are all hydrolysed.
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CAS REGISTRY NUMBER
COMMENTARY
884593-92-4
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9001-22-3
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(R)-1-phenylethyl beta-D-glucopyranoside + H2O
(R)-1-phenylethanol + beta-D-glucopyranose
LC-MS analysis and quantitative GC-MS analysis of substrate and product
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?
(R)-1-phenylethyl beta-primeveroside + H2O
(R)-1-phenylethanol + beta-primeverose
LC-MS analysis and quantitative GC-MS analysis of substrate and product
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?
(R)-linalyl beta-vicianoside + H2O
linalol + 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside
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?
(S)-1-phenylethyl beta-D-glucopyranoside + H2O
(S)-1-phenylethanol + beta-D-glucopyranose
LC-MS analysis and quantitative GC-MS analysis of substrate and product
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?
(S)-1-phenylethyl beta-primeveroside + H2O
(S)-1-phenylethanol + beta-primeverose
LC-MS analysis and quantitative GC-MS analysis of substrate and product
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?
2-phenylethyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
2-phenylethyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
2-phenylethyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranose + 2-phenylethanol
pH 6.0, 37°C, 0.05% activity
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-
?
2-phenylethyl 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O
beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranose + 2-phenylethanol
pH 6.0, 37°C, 0.60% activity
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-
?
2-phenylethyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
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trivial name beta-primeveroside
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?
2-phenylethyl beta-acuminoside + H2O
2-phenylethanol + 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside
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i.e. 2-phenylethyl 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside
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?
2-phenylethyl beta-D-glucopyranoside + H2O
2-phenylethanol + beta-D-glucopyranose
low activity
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?
2-phenylethyl beta-D-glucopyranoside + H2O
2-phenylethanol + D-glucopyranose
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?
2-phenylethyl beta-gentiobioside + H2O
gentiobiose + 2-phenylethanol
pH 6.0, 37°C, 0.34% activity
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?
2-phenylethyl beta-primeveroside + H2O
2-phenylethanol + beta-primeverose
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?
2-phenylethyl beta-vicianoside + H2O
2-phenylethanol + 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside
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?
2-phenylethyl beta-vicianoside + H2O
vicianose + 2-phenylethanol
pH 6.0, 37°C, 2.97% activity
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?
2-phenylethyl-6-O-alpha-D-arabinosyl-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-alpha-D-arabinosyl-beta-D-glucopyranoside
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trivial name beta-vicianoside, 3% of activity with 2-phenyl-beta-primeveroside
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?
2-phenylethyl-6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside
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trivial name beta-vicianoside, 0.8% of activity with 2-phenyl-beta-primeveroside
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?
2-phenylethyl-6-O-beta-D-glucopyranosyl-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-beta-D-glucopyranosyl-beta-D-glucopyranoside
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trivial name beta-vicianoside, 0.25% of activity with 2-phenyl-beta-primeveroside
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?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
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?
4-nitrophenyl beta-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
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pH 5.5, 40°C, 15% hydrolysis
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?
4-nitrophenyl beta-primeveroside + H2O
4-nitrophenol + primeverose
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i.e. 4-nitrophenyl-6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
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?
4-nitrophenyl-beta-primeveroside + H2O
4-nitrophenol + beta-primeverose
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?
6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside + H2O
alpha-L-arabinopyranose + beta-D-glucopyranose
hydrolyzed with lower activity
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?
6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O
beta-D-apiofuranose + beta-D-glucopyranose
hydrolyzed with lower activity
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?
6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O
beta-D-xylopyranose + beta-D-glucopyranose
hydrolyzed with highest activity
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?
6-O-beta-L-arabinopyranosyl-beta-D-glucopyranoside + H2O
beta-D-arabinopyranose + beta-D-glucopyranose
hydrolyzed with lower activity
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?
a 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose + an alcohol
a beta-primeveroside + H2O
? + primeverose
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the enzyme shows broad substrate specificity with respect to the disaccharide glycon moiety (subsite-2), Thr271 and Thr415 play important roles in subsite-2 of the enzyme
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?
benzyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
benzyl alcohol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
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i.e. 2-benzyl beta-D-primeveroside
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?
benzyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
benzyl alcohol + 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside
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?
benzyl 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O
benzyl alcohol + beta-D-apiofuranosyl-(1->6)-D-glucose
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?
geranyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
geraniol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
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i.e. geranyl beta-D-primeveroside
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?
geranyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
geraniol + 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside
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?
geranyl beta-primeveroside + H2O
geraniol + primeverose
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pH 5.5, 40°C, 0.1% hydrolysis
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?
linalyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
linalol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
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?
linalyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
linalool + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
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?
p-nitrophenol beta-D-glucopyranoside + H2O
D-glucose + p-nitrophenol
pH 6.0, 37°C, 0.23% activity
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?
p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O
p-nitrophenol + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
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trivial name beta-primeveroside
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?
p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O
primeverose + p-nitrophenol
p-nitrophenyl beta-D-glucoside + H2O
p-nitrophenol + D-glucose
pH 6.0, 37°C, 0.5% activity (purified from tea), 0.3% activity (recombinant enzyme)
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?
p-nitrophenyl beta-gentiobioside + H2O
p-nitrophenol + D-gentiobiose
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pH 5.5, 40°C, 2.5% hydrolysis
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?
p-nitrophenyl beta-gentiobioside + H2O
p-nitrophenol + gentiobiose
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37°C, 41% hydrolysis
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?
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
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?
p-nitrophenyl-beta-D-glucopyranoside + H2O
p-nitrophenol + beta-D-glucose
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?
(Z)-3-hexenyl beta-primeveroside + H2O
(Z)-3-hexenol + primeverose
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pH 5.5, 40°C, 0.1% hydrolysis
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?
(Z)-3-hexenyl beta-primeveroside + H2O
(Z)-3-hexenol + primeverose
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pH 5.5, 40°C, 0.1% hydrolysis
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?
2-phenylethyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
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i.e. 2-phenylethyl beta-D-primeveroside
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?
2-phenylethyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
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i.e. 2-phenylethyl beta-D-primeveroside
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?
2-phenylethyl beta-primeveroside + H2O
2-phenylethanol + primeverose
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pH 5.5, 40°C, 0.1% hydrolysis
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?
2-phenylethyl beta-primeveroside + H2O
2-phenylethanol + primeverose
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pH 5.5, 40°C, 0.1% hydrolysis
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?
2-phenylethyl beta-primeveroside + H2O
primeverose + 2-phenylethanol
pH 6.0, 37°C
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?
2-phenylethyl beta-primeveroside + H2O
primeverose + 2-phenylethanol
pH 6.0, 37°C, 100% activity
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?
2-phenylethyl beta-primeveroside + H2O
primeverose + 2-phenylethanol
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pH 6.0, 37°C, 100% activity
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?
a 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose + an alcohol
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?
a 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose + an alcohol
the enzyme hydrolyzes beta-primeveroside at the beta-glycosidic bond of primeverose to aglycone, and releases aromatic alcoholic volatiles of aglycones
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?
a 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose + an alcohol
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?
benzyl beta-primeveroside + H2O
phenylmethanol + primeverose
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pH 5.5, 40°C, 0.1% hydrolysis
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?
benzyl beta-primeveroside + H2O
phenylmethanol + primeverose
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pH 5.5, 40°C, 0.1% hydrolysis
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?
eugenyl beta-primeveroside + H2O
eugenol + primeverose
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37°C, 1.8% hydrolysis
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?
eugenyl beta-primeveroside + H2O
eugenol + primeverose
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37°C, 1.8% hydrolysis
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?
eugenyl beta-primeveroside + H2O
eugenol + primeverose
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i.e. eugenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
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?
eugenyl beta-primeveroside + H2O
eugenol + primeverose
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pH 5.5, 40°C, 2.5% hydrolysis
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?
eugenyl beta-primeveroside + H2O
eugenol + primeverose
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pH 5.5, 40°C, 2.5% hydrolysis
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?
furcatin + H2O
4-allyl-phenol + ?
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pH 5.5, 40°C, 1.0% hydrolysis
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?
furcatin + H2O
4-allyl-phenol + ?
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pH 5.5, 40°C, 1.0% hydrolysis
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?
p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O
primeverose + p-nitrophenol
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37°C, 100% hydrolysis
-
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?
p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O
primeverose + p-nitrophenol
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37°C, 100% hydrolysis
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-
?
p-nitrophenyl beta-primeveroside + H2O
primeverose + p-nitrophenol
pH 6.0, 37°C
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-
?
p-nitrophenyl beta-primeveroside + H2O
primeverose + p-nitrophenol
pH 6.0, 37°C, 48% activity (purified from tea), 62% activity (recombinant enzyme)
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-
?
p-nitrophenyl beta-primeveroside + H2O
primeverose + p-nitrophenol
pH 6.0, 37°C, 54.4% activity
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?
p-nitrophenyl beta-primeveroside + H2O
primeverose + p-nitrophenol
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pH 5.5, 40°C, 100% hydrolysis
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?
additional information
?
-
no activity with p-nitrophenol beta-D-xyloside and 2-phenylethyl beta-D-glucoside
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?
additional information
?
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no activity with p-nitrophenol beta-D-xyloside and 2-phenylethyl beta-D-glucoside
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?
additional information
?
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does not act on 2-phenylethyl beta-D-glucopyranoside
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?
additional information
?
-
the enzyme only accepts primeverose as the glycone substrate, but broadly accepts various aglycones, including 2-phenylethanol, benzyl alcohol, linalool, and geraniol. Compared with aglycone-specific beta-glucosidases of the glycoside hydrolase family 1, beta-primeverosidase lacks the Trp crucial for aglycone recognition, and the resultant large cavity accepts aglycone and 6-O-beta-D-xylopyranosyl together. The enzyme barely hydrolyzes other beta-glycosides, in which the beta-1,6-Xyl group is absent or substituted by other sugars, such as monosaccharide glycosides (beta-D-glucopyranoside and beta-D-xylopyranoside, etc.) or other disaccharide glycosides, beta-vicianosides (6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside), beta-gentiobiosides (6-O-beta-D-glucopyranosyl-beta-D-glucopyranoside), and beta-acuminosides (6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside)
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-
?
additional information
?
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-
the diglycoside-specific enzyme beta-primeverosidase catalyses the hydrolysis of primeveroside into primeverose (a disaccharide of Xylbeta1-6Glc) and volatile aglycons
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-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(R)-linalyl beta-vicianoside + H2O
linalol + 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside
-
-
-
?
2-phenylethyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
2-phenylethyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
-
trivial name beta-primeveroside
-
-
?
2-phenylethyl beta-vicianoside + H2O
2-phenylethanol + 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside
-
-
-
?
a 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose + an alcohol
benzyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
benzyl alcohol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
-
i.e. 2-benzyl beta-D-primeveroside
-
?
eugenyl beta-primeveroside + H2O
eugenol + primeverose
-
i.e. eugenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
-
-
?
geranyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
geraniol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
-
i.e. geranyl beta-D-primeveroside
-
?
geranyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
geraniol + 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside
-
-
-
?
linalyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
linalol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
-
-
-
?
additional information
?
-
-
the diglycoside-specific enzyme beta-primeverosidase catalyses the hydrolysis of primeveroside into primeverose (a disaccharide of Xylbeta1-6Glc) and volatile aglycons
-
-
?
2-phenylethyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
-
i.e. 2-phenylethyl beta-D-primeveroside
-
?
2-phenylethyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
-
i.e. 2-phenylethyl beta-D-primeveroside
-
?
a 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose + an alcohol
-
-
-
?
a 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose + an alcohol
-
-
-
-
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-phenyl-N-(6-O-beta-D-xylopyranosyl-beta-D-glucopyranosyl)ethylamidine
enzyme binding structure, overview
4-(N-benzylsuccinimide-3-sulfanyl)-N-(6-O-beta-D-xylopyranosyl-beta-D-glucopyranosyl)butylamidine
enzyme binding structure, overview
N-(2-phenylethanimidoyl)-6-O-beta-D-xylopyranosyl-beta-D-glucopyranosylamine
strongly inhibits
N-[4-[(1-benzyl-2,5-dioxopyrrolidin-3-yl)sulfanyl]butanimidoyl]-6-O-beta-D-xylopyranosyl-beta-D-glucopyranosylamine
potent inhibitor
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Acidosis
Acidosis and increased plasma levels of beta-D-glucosidase and beta-D-galactosidase after hepatic inflow occlusion in the pig.
Arthritis, Experimental
[Activity of acid hydrolases in rat tissues under experimental arthritis]
Breast Neoplasms
Serum beta-N-acetylglucosaminidase, beta-D-glucosidase, alpha-D-glucosidase, beta-D-fucosidase, alpha-L-fucosidase and beta-D-galactosidase levels in acute viral hepatitis, pancreatitis, myocardial infarction and breast cancer.
Carcinoma
Studies of the activities of lysosomal enzymes in serum and buccal pouch tissue of hamsters during 7,12-dimethylbenz[a]anthracene-induced carcinogenesis.
Carcinoma
Studies on variations of glycoproteins and lysosomal hydrolases in human uterine cervical carcinoma.
Cataract
Variation in glycosidase activity in soluble fractions in ICR/f rat lenses with the progression of cataract formation.
Coccidiosis
[beta-D-glucosidase in the microvillous zone of small intestine enterocytes in experimental coccidiosis in suckling piglets]
Colitis
In vitro evaluation of dexamethasone-beta-D-glucuronide for colon-specific drug delivery.
Crohn Disease
Bacterial enzymes used for colon-specific drug delivery are decreased in active Crohn's disease.
Diabetes Mellitus
Alterations in the activity of several glycohydrolases in red blood cell membrane from type 2 diabetes mellitus patients.
Diabetes Mellitus, Type 1
Prospective study of the enzymatic activities in urine of N-acetyl-beta-D-glucosaminidase, alpha-D-mannosidase, alpha- and beta-D-glucosidases, alpha-L- and beta-D-fucosidases, and beta-D-galactosidase in type I diabetes mellitus with early nephropathy.
Diabetes Mellitus, Type 2
A TLC bioautographic method for the detection of alpha- and beta-glucosidase inhibitors in plant extracts.
Diabetes Mellitus, Type 2
Alterations in the activity of several glycohydrolases in red blood cell membrane from type 2 diabetes mellitus patients.
Diabetic Nephropathies
Prospective study of the enzymatic activities in urine of N-acetyl-beta-D-glucosaminidase, alpha-D-mannosidase, alpha- and beta-D-glucosidases, alpha-L- and beta-D-fucosidases, and beta-D-galactosidase in type I diabetes mellitus with early nephropathy.
Gangliosidosis, GM1
Multiple carbohydrate-cleaving specificities in human acidic and neutral glycosidases.
Gaucher Disease
Beta-glucosidase activity in liver, spleen and brain in acute neuropathic Gaucher disease.
Gaucher Disease
Marked increase of methylumbelliferyl-tetra-N-acetylchitotetraoside hydrolase activity in plasma from Gaucher disease patients.
Gaucher Disease
Multiple carbohydrate-cleaving specificities in human acidic and neutral glycosidases.
Hepatitis
Serum beta-N-acetylglucosaminidase, beta-D-glucosidase, alpha-D-glucosidase, beta-D-fucosidase, alpha-L-fucosidase and beta-D-galactosidase levels in acute viral hepatitis, pancreatitis, myocardial infarction and breast cancer.
Infections
[beta-D-glucosidase in the microvillous zone of small intestine enterocytes in experimental coccidiosis in suckling piglets]
Myocardial Infarction
Serum beta-N-acetylglucosaminidase, beta-D-glucosidase, alpha-D-glucosidase, beta-D-fucosidase, alpha-L-fucosidase and beta-D-galactosidase levels in acute viral hepatitis, pancreatitis, myocardial infarction and breast cancer.
Neoplasms
Studies on variations of glycoproteins and lysosomal hydrolases in human uterine cervical carcinoma.
Neoplasms
[Detection and distribution of the hydrolytic enzymes beta-D-glucuronidase, beta-D-galactosidase, beta-D-glucosidase and arylsulfatase in intracranial tumors]
Neurilemmoma
[Demonstration and distribution of the enzymes: beta-D-glucuronidase, beta-D-galactosidase, beta-D-glucosidase, and arylsulfatase in neurinomas.]
Pancreatitis
Serum beta-N-acetylglucosaminidase, beta-D-glucosidase, alpha-D-glucosidase, beta-D-fucosidase, alpha-L-fucosidase and beta-D-galactosidase levels in acute viral hepatitis, pancreatitis, myocardial infarction and breast cancer.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2
2-phenylethyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside
-
37°C, pH 6.0
14.9
2-phenylethyl-6-O-alpha-D-arabinosyl-beta-D-glucopyranoside
-
37°C, pH 6.0
2.9
p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside
-
37°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
43.7
2-phenylethyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside
-
37°C, pH 6.0
5.85
2-phenylethyl-6-O-alpha-D-arabinosyl-beta-D-glucopyranoside
-
37°C, pH 6.0
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.026
4-(N-benzylsuccinimide-3-sulfanyl)-N-(6-O-beta-D-xylopyranosyl-beta-D-glucopyranosyl)butylamidine
recombinant His-tagged enzyme, pH and temperature not specified in the publication
0.14
N-(2-phenylethanimidoyl)-6-O-beta-D-xylopyranosyl-beta-D-glucopyranosylamine
-
0.026
N-[4-[(1-benzyl-2,5-dioxopyrrolidin-3-yl)sulfanyl]butanimidoyl]-6-O-beta-D-xylopyranosyl-beta-D-glucopyranosylamine
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.9
0.98
measured with p-nitrophenol beta-primeveroside in 20 mM citrate buffer (pH 6.0) at 37°C
0.99
measured with p-nitrophenol beta-primeveroside in 20 mM citrate buffer (pH 6.0) at 37°C
1.7
0.9
measured with p-nitrophenol beta-primeveroside in 20 mM citrate buffer (pH 6.0) at 37°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
45
55
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50 - 60
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
var assamica