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Literature summary for 3.2.1.149 extracted from

  • Yamamoto, S.; Okada, M.; Usui, T.; Sakata, K.
    Isolation and characterization of a beta-primeverosidase-like endo-manner beta-glycosidase from Aspergillus fumigatus AP-20 (2002), Biosci. Biotechnol. Biochem., 66, 801-807.
    View publication on PubMed
Search for more literature for 3.2.1.149

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.9
-
 p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside 37°C Aspergillus fumigatus
6.3
-
 p-nitrophenyl beta-gentiobioside 37°C Aspergillus fumigatus
6.3
-
 eugenyl beta-primeveroside 37°C Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 x * 47000, SDS-PAGE Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
 AP-20
-
Aspergillus fumigatus AP-20
-
 AP-20
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus fumigatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4
-
 purified enzyme Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + H2O 37°C, 0.6% hydrolysis Aspergillus fumigatus 2 D-glucose
-
 ?
cellobiose + H2O 37°C, 0.6% hydrolysis Aspergillus fumigatus AP-20 2 D-glucose
-
 ?
eugenyl beta-primeveroside + H2O 37°C, 1.8% hydrolysis Aspergillus fumigatus eugenol + primeverose
-
 ?
eugenyl beta-primeveroside + H2O 37°C, 1.8% hydrolysis Aspergillus fumigatus AP-20 eugenol + primeverose
-
 ?
gentiobiose + H2O 37°C, 5.1% hydrolysis Aspergillus fumigatus D-glucose
-
 ?
gentiobiose + H2O 37°C, 5.1% hydrolysis Aspergillus fumigatus AP-20 D-glucose
-
 ?
laminaribiose + H2O 37°C, 1.6% hydrolysis Aspergillus fumigatus D-glucose
-
 ?
laminaribiose + H2O 37°C, 1.6% hydrolysis Aspergillus fumigatus AP-20 D-glucose
-
 ?
p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O 37°C, 100% hydrolysis Aspergillus fumigatus primeverose + p-nitrophenol
-
 ?
p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O 37°C, 100% hydrolysis Aspergillus fumigatus AP-20 primeverose + p-nitrophenol
-
 ?
p-nitrophenyl beta-gentiobioside + H2O 37°C, 41% hydrolysis Aspergillus fumigatus p-nitrophenol + gentiobiose
-
 ?
primeverose + H2O 37°C, 0.2% hydrolysis Aspergillus fumigatus D-xylose + D-glucose
-
 ?
sophorose + H2O 37°C, 0.5% hydrolysis Aspergillus fumigatus 2 D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 47000, SDS-PAGE Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
beta-primeverosidase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Aspergillus fumigatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 60 more than 90% of optimum activity Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5 3
-
 Aspergillus fumigatus

pH Range

pH Minimum pH Maximum Comment Organism
7 8
-
 Aspergillus fumigatus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus fumigatus isoelectric focusing
-
 6