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Literature summary for 3.2.1.130 extracted from

  • Iwamoto, S.; Kasahara, Y.; Yoshimura, Y.; Seko, A.; Takeda, Y.; Ito, Y.; Totani, K.; Matsuo, I.
    Endo-alpha-mannosidase-catalyzed transglycosylation (2017), ChemBioChem, 18, 1376-1378 .
    View publication on PubMed
Search for more literature for 3.2.1.130

Application

Application Comment Organism
synthesis the mutant has excellent transglycosylation activity and extremely low hydrolytic activity. The minimum motif required for glycosyl acceptor is Man-alpha-(1->2)Man. The synthetic utility of the enzyme is demonstrated by generation of a high-mannose-type undecasaccharide (Glc1Man9GlcNAc2) Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expresion in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
E407D the mutant has excellent transglycosylation activity and extremely low hydrolytic activity. The minimum motif required for glycosyl acceptor is Man-alpha-(1->2)-Man Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q5SRI9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is able to mediate transglycosylation from alpah-glycosyl-fluorides Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
Golgi endo-a-mannosidase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 optimum for transglycosylation Homo sapiens