Literature summary for 3.2.1.114 extracted from

Kawatkar, S.P.; Kuntz, D.A.; Woods, R.J.; Rose, D.R.; Boons, G.J.
Structural basis of the inhibition of Golgi alpha-mannosidase II by mannostatin A and the role of the thiomethyl moiety in ligand-protein interactions (2006), J. Am. Chem. Soc., 128, 8310-8319.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in complex with inhibitors mannostatin A, N-benzyl mannostatin A, and 1-deoxyaminocyclopentitetrol, crystal growth overnight of enzyme solved in phosphate buffered reservoir solution, crystals are soaked for 3-6 h in 10 mM ligand solution, co-crystallization in Tris buffer without phosphate washing, X-ray diffraction structure determination and analysis, molecular modelling and structure simulation, overview	Drosophila melanogaster

Crystallization (Comment)

Organism

enzyme in complex with inhibitors mannostatin A, N-benzyl mannostatin A, and 1-deoxyaminocyclopentitetrol, crystal growth overnight of enzyme solved in phosphate buffered reservoir solution, crystals are soaked for 3-6 h in 10 mM ligand solution, co-crystallization in Tris buffer without phosphate washing, X-ray diffraction structure determination and analysis, molecular modelling and structure simulation, overview

Drosophila melanogaster

Inhibitors

Inhibitors	Comment	Organism
1-deoxyamino-cyclopentitetrol	-	Drosophila melanogaster
1-deoxyaminocyclopentitetrol	structural basis of the inhibition of Golgi alpha-mannosidase II and the role of the thiomethyl moiety in ligand-protein interactions, overview	Drosophila melanogaster
Mannostatin A	structural basis of the inhibition of Golgi alpha-mannosidase II and the role of the thiomethyl moiety in ligand-protein interactions, overview	Drosophila melanogaster
additional information	simulations of ligand/inhibitor binding	Drosophila melanogaster
N-benzyl mannostatin A	structural basis of the inhibition of Golgi alpha-mannosidase II and the role of the thiomethyl moiety in ligand-protein interactions, overview	Drosophila melanogaster
N-benzyl-mannostatin	-	Drosophila melanogaster

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi apparatus	Golgi alpha-mannosidase II	Drosophila melanogaster	5794	-

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
method not mentioned	Drosophila melanogaster

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl alpha-D-mannoside + H2O	-	Drosophila melanogaster	4-methylumbelliferol + alpha-D-mannose	-	?

Synonyms

Synonyms	Comment	Organism
GmII	-	Drosophila melanogaster
Golgi alpha-mannosidase II	-	Drosophila melanogaster

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.000036	-	Mannostatin A	-	Drosophila melanogaster
0.000249	-	N-benzyl mannostatin A	-	Drosophila melanogaster
0.000249	-	N-benzyl-mannostatin	-	Drosophila melanogaster
0.265	-	1-deoxyaminocyclopentitetrol	-	Drosophila melanogaster
0.265	-	1-deoxyamino-cyclopentitetrol	-	Drosophila melanogaster