Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates, responsible for regulation of substrate specificity | Ginkgo biloba |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
x * 120000, SDS-PAGE | Ginkgo biloba |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Ginkgo biloba | Co2+ regulates the substrate specificity, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ginkgo biloba | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from mature seeds to homogeneity by ammonium sulfate fractionation, ion exchange and hydrophobic interaction chromatography, and gel filtration | Ginkgo biloba |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Ginkgo biloba | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-alpha-D-mannoside + H2O | - |
Ginkgo biloba | 4-nitrophenol + alpha-D-mannose | - |
? | |
Manalpha(1-3)Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O | - |
Ginkgo biloba | Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose | - |
? | |
Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O | - |
Ginkgo biloba | Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose | - |
? | |
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O | - |
Ginkgo biloba | Manalpha(1-3)Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose | - |
? | |
Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O | - |
Ginkgo biloba | Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose | - |
? | |
additional information | Co2+ regulates the substrate specificity, overview | Ginkgo biloba | ? | - |
? | |
additional information | in vitro hydrolysis of high-mannose type N-glycans, removal of terminal alpha-1,2-mannose residues, substrate specificity and isomeric substrate/product structures, overview | Ginkgo biloba | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 120000, SDS-PAGE | Ginkgo biloba |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Ginkgo biloba |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Ginkgo biloba |