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Literature summary for 3.2.1.114 extracted from
- alpha-Mannosidases I and II from Aspergillus saitoi (1987), Methods Enzymol., 138, 779-785.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.25 | - |
Manalpha(1,6)(Manalpha(1,3))Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc | NaBH4 reduced | Aspergillus phoenicis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | strongly activates, 1 mM, 13fold | Aspergillus phoenicis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus phoenicis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | freeze-dried mixture of mycelium and its culture medium, commercially available as Morushin | Aspergillus phoenicis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
- |
Aspergillus phoenicis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C or 4°C, 10 mM sodium acetate buffer, pH 5, with or without CaCl2, 4 months, stable | Aspergillus phoenicis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O | NaBH4 reduced, poor substrate | Aspergillus phoenicis | ? | - |
? | |
| D-Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O | NaBH4 reduced, good substrate | Aspergillus phoenicis | D-Manbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
| Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Aspergillus phoenicis | Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
| additional information | not: 4-nitrophenyl alpha-mannoside | Aspergillus phoenicis | ? | - |
? | |
| additional information | not: Manalpha(1-6)(R-Manalpha(1-3))Manbeta(1-4)GlcNAc | Aspergillus phoenicis | ? | - |
? | |
| R-Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O | - |
Aspergillus phoenicis | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Aspergillus phoenicis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus phoenicis |
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Release 2023.1 (January 2023)
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