Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | the intracellular localization is changed depending on the growth phase. Distributed in the cytosol until the initial logarithmic phase (12 h), then delivered to an organelle with a granule structure in the middle logarithmic phase (14 h), and finally localized at the vacuole membranes in the stationary phase (18-20 h) | Saccharomyces cerevisiae | 5829 | - |
membrane | the intracellular localization is changed depending on the growth phase. Distributed in the cytosol until the initial logarithmic phase (12 h), then delivered to an organelle with a granule structure in the middle logarithmic phase (14 h), and finally localized at the vacuole membranes in the stationary phase (18-20 h) | Saccharomyces cerevisiae | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
138000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P40566 | - |
- |
Saccharomyces cerevisiae ATCC 204508 | P40566 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae | D-glucose + 4-methylumbelliferone | - |
? | |
4-methylumbelliferyl beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae ATCC 204508 | D-glucose + 4-methylumbelliferone | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae | D-glucose + 4-nitrophenol | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae ATCC 204508 | D-glucose + 4-nitrophenol | - |
? | |
cholesteryl 3-O-beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae | D-glucose + cholesterol | - |
? | |
cholesteryl 3-O-beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae ATCC 204508 | D-glucose + cholesterol | - |
? | |
ergosteryl 3-O-beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae | D-glucose + ergosterol | - |
? | |
ergosteryl 3-O-beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae ATCC 204508 | D-glucose + ergosterol | - |
? | |
glucosylceramide + H2O | - |
Saccharomyces cerevisiae | D-glucose + ceramide | - |
? | |
glucosylceramide + H2O | - |
Saccharomyces cerevisiae ATCC 204508 | D-glucose + ceramide | - |
? | |
sitosteryl 3-O-beta-D-glucoside + H2O | - |
Saccharomyces cerevisiae | D-glucose + sitosterol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 138000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Egh1 | - |
Saccharomyces cerevisiae |
ergosteryl-beta-glucosidase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | the disruption of EGH1 in Saccharomyces cerevisiae BY4741 (egh1DELTA) results in the accumulation of ergosteryl 3-O-beta-glucoside and fragmentation of vacuoles | Saccharomyces cerevisiae |
physiological function | involved in sterylglucoside catabolism and vacuole formation | Saccharomyces cerevisiae |