Information on EC 3.2.1.104 - steryl-beta-glucosidase

for references in articles please use BRENDA:EC3.2.1.104
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The expected taxonomic range for this enzyme is: Sinapis alba

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EC NUMBER
COMMENTARY hide
3.2.1.104
-
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RECOMMENDED NAME
GeneOntology No.
steryl-beta-glucosidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cholesteryl-beta-D-glucoside + H2O = D-glucose + cholesterol
show the reaction diagram
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
cholesteryl-beta-D-glucoside glucohydrolase
Acts on glucosides of cholesterol and sitosterol, but not on some related sterols such as coprostanol.
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CAS REGISTRY NUMBER
COMMENTARY hide
69494-88-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cholesteryl beta-D-glucoside + H2O
D-glucose + cholesterol
show the reaction diagram
-
-
-
-
?
p-nitrophenyl beta-D-glucoside + H2O
D-glucose + p-nitrophenol
show the reaction diagram
-
-
-
-
?
sitosteryl beta-D-glucoside + H2O
D-glucose + sitosterol
show the reaction diagram
-
-
-
-
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-lyso-phosphatidylcholine
-
-
2-lyso-phosphatidylethanolamine
-
-
5alpha-cholestanol
-
hydrolysis of cholesteryl glucoside
campesterol
-
hydrolysis of cholesteryl glucoside
cholesterol
-
hydrolysis of cholesteryl glucoside
ethanol
-
39% inhibition at a concentration of 50%, hydrolysis of p-nitrophenyl beta-D-glucoside
gluconolactone
-
50% inhibition at 4 mM, hydrolysis of cholesteryl beta-D-glucoside. 50% inhibition at 2 mM, hydrolysis of p-nitrophenyl beta-D-glucoside
monogalactosyldiacylglycerol
-
-
phosphatidylcholine
-
-
phosphatidylethanolamine
-
-
sitosterol
-
hydrolysis of cholesteryl glucoside
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cholesteryl acetate
-
150% stimulation at 0.1 mM
dithiothreitol
-
45% stimulation at 0.1 mM, hydrolysis of cholesteryl beta-D-glucoside. No effect on hydrolysis of p-nitrophenyl beta-D-glucoside
ethanol
-
64% stimulation at a concentration of 20%, hydrolysis of cholesteryl beta-D-glucoside. 65% stimulation at a concentration of 20%, hydrolysis of p-nitrophenyl beta-D-glucoside
methyl stearate
-
150% stimulation at 0.1 mM
phytol
-
200% stimulation at 0.002 mM
Tripalmitoylglycerol
-
145% stimulation at 0.1 mM
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.3621
-
-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2 - 5.6
-
hydrolysis of cholesteryl beta-D-glucoside or p-nitrophenyl beta-D-glucoside
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
hydrolysis of cholesteryl beta-D-glucoside
60
-
hydrolysis of p-nitrophenyl beta-D-glucoside
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65000
-
gel filtration
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
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LINKS TO OTHER DATABASES (specific for EC-Number 3.2.1.104)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)