We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 3.2.1.104 - steryl-beta-glucosidase for references in articles please use BRENDA:EC3.2.1.104
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Acts on glucosides of cholesterol and sitosterol, but not on some related sterols such as coprostanol.
The enzyme appears in viruses and cellular organisms
Synonyms
EGCrP2, Egh1, endoglycoceramidase-related protein 2, ergosteryl-beta-glucosidase, glucosidase, hydroxy steroid beta-, SG hydrolase, steryl beta-D-glucoside hydrolase, steryl glucoside hydrolase,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
endoglycoceramidase-related protein 2
ergosteryl-beta-glucosidase
glucosidase, hydroxy steroid beta-
-
-
-
-
steryl beta-D-glucoside hydrolase
-
-
-
-
steryl glucoside hydrolase
-
-
-
-
EGCrP2
-
Egh1
-
endoglycoceramidase-related protein 2
-
endoglycoceramidase-related protein 2
-
-
ergosteryl-beta-glucosidase
-
ergosteryl-beta-glucosidase
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cholesteryl-beta-D-glucoside + H2O = D-glucose + cholesterol
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hydrolysis of O-glycosyl bond
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cholesteryl-beta-D-glucoside glucohydrolase
Acts on glucosides of cholesterol and sitosterol, but not on some related sterols such as coprostanol.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide + H2O
D-glucose + 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanol
4-methylumbelliferyl beta-D-glucoside + H2O
D-glucose + 4-methylumbelliferone
4-nitrophenyl beta-D-glucoside + H2O
D-glucose + 4-nitrophenol
arbutin + H2O
D-glucose + benzene-1,4-diol
-
-
-
?
cholesteryl 3-O-beta-D-glucoside + H2O
D-glucose + cholesterol
cholesteryl 3-O-beta-glucoside + H2O
D-glucose + cholesterol
-
-
-
?
cholesteryl beta-D-glucoside + H2O
D-glucose + cholesterol
-
-
-
-
?
ergosteryl 3-O-beta-D-glucoside + H2O
D-glucose + ergosterol
ergosteryl 3-O-beta-glucoside + H2O
D-glucose + erosterol
-
-
-
?
glucosylceramide + H2O
D-glucose + ceramide
indoxyl beta-D-glucoside + H2O
D-glucose + indoxyl
-
-
-
?
n-octyl beta-D-glucoside + H2O
D-glucose + n-octanol
-
-
-
?
p-nitrophenyl beta-D-glucoside + H2O
D-glucose + p-nitrophenol
-
-
-
-
?
phloridzin + H2O
D-glucose + phloretin
-
-
-
?
resorufin 3-O-beta-D-glucoside + H2O
D-glucose + resorufin
-
-
-
?
salicin + H2O
D-glucose + 2-(hydroxymethyl)phenol
-
-
-
?
sitosteryl 3-O-beta-D-glucoside + H2O
D-glucose + sitosterol
-
-
-
?
sitosteryl 3-O-beta-glucoside + H2O
D-glucose + sitosterol
-
-
-
?
sitosteryl beta-D-glucoside + H2O
D-glucose + sitosterol
-
-
-
-
?
additional information
?
-
(6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide + H2O
D-glucose + 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanol
-
-
-
?
(6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide + H2O
D-glucose + 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanol
-
-
-
?
4-methylumbelliferyl beta-D-glucoside + H2O
D-glucose + 4-methylumbelliferone
-
-
-
?
4-methylumbelliferyl beta-D-glucoside + H2O
D-glucose + 4-methylumbelliferone
-
-
-
?
4-methylumbelliferyl beta-D-glucoside + H2O
D-glucose + 4-methylumbelliferone
-
-
-
?
4-methylumbelliferyl beta-D-glucoside + H2O
D-glucose + 4-methylumbelliferone
-
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
D-glucose + 4-nitrophenol
-
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
D-glucose + 4-nitrophenol
-
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
D-glucose + 4-nitrophenol
-
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
D-glucose + 4-nitrophenol
-
-
-
?
cholesteryl 3-O-beta-D-glucoside + H2O
D-glucose + cholesterol
-
-
-
?
cholesteryl 3-O-beta-D-glucoside + H2O
D-glucose + cholesterol
-
-
-
?
ergosteryl 3-O-beta-D-glucoside + H2O
D-glucose + ergosterol
-
-
-
?
ergosteryl 3-O-beta-D-glucoside + H2O
D-glucose + ergosterol
-
-
-
?
glucosylceramide + H2O
D-glucose + ceramide
-
-
-
?
glucosylceramide + H2O
D-glucose + ceramide
-
-
-
?
glucosylceramide + H2O
D-glucose + ceramide
-
-
-
?
glucosylceramide + H2O
D-glucose + ceramide
-
-
-
?
additional information
?
-
no activity with alpha-glucosides or beta-galactosides, under acidic conditions
-
-
-
additional information
?
-
no activity with alpha-glucosides or beta-galactosides, under acidic conditions
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-lyso-phosphatidylcholine
-
-
2-lyso-phosphatidylethanolamine
-
-
5alpha-cholestanol
-
hydrolysis of cholesteryl glucoside
campesterol
-
hydrolysis of cholesteryl glucoside
cholesterol
-
hydrolysis of cholesteryl glucoside
ethanol
-
39% inhibition at a concentration of 50%, hydrolysis of p-nitrophenyl beta-D-glucoside
gluconolactone
-
50% inhibition at 4 mM, hydrolysis of cholesteryl beta-D-glucoside. 50% inhibition at 2 mM, hydrolysis of p-nitrophenyl beta-D-glucoside
monogalactosyldiacylglycerol
-
-
phosphatidylethanolamine
-
-
sitosterol
-
hydrolysis of cholesteryl glucoside
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Cholesteryl acetate
-
150% stimulation at 0.1 mM
dithiothreitol
-
45% stimulation at 0.1 mM, hydrolysis of cholesteryl beta-D-glucoside. No effect on hydrolysis of p-nitrophenyl beta-D-glucoside
ethanol
-
64% stimulation at a concentration of 20%, hydrolysis of cholesteryl beta-D-glucoside. 65% stimulation at a concentration of 20%, hydrolysis of p-nitrophenyl beta-D-glucoside
methyl stearate
-
150% stimulation at 0.1 mM
phytol
-
200% stimulation at 0.002 mM
Tripalmitoylglycerol
-
145% stimulation at 0.1 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0384
(6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide
pH 6.0, 30°C
0.034
4-methylumbelliferyl beta-D-glucoside
pH 6.0, 30°C
0.817
4-nitrophenyl beta-D-glucoside
pH 6.0, 30°C
0.0436
resorufin 3-O-beta-D-glucoside
pH 6.0, 30°C
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.53
(6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide
pH 6.0, 30°C
8.8
4-methylumbelliferyl beta-D-glucoside
pH 6.0, 30°C
13.8
4-nitrophenyl beta-D-glucoside
pH 6.0, 30°C
21.9
resorufin 3-O-beta-D-glucoside
pH 6.0, 30°C
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
13.8
(6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide
pH 6.0, 30°C
25.7
4-methylumbelliferyl beta-D-glucoside
pH 6.0, 30°C
16.9
4-nitrophenyl beta-D-glucoside
pH 6.0, 30°C
504
resorufin 3-O-beta-D-glucoside
pH 6.0, 30°C
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5.2 - 5.6
-
hydrolysis of cholesteryl beta-D-glucoside or p-nitrophenyl beta-D-glucoside
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4 - 7
pH 4.0: about % of maximal activity, pH 7.0: about % of maximal activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
60
-
hydrolysis of p-nitrophenyl beta-D-glucoside
30
assay at
30
-
hydrolysis of cholesteryl beta-D-glucoside
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
16 - 40
16°C: about 50% of maximal activity, 40°C: about 75% of maximal activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
UniProt
brenda
-
UniProt
brenda
-
SwissProt
brenda
-
SwissProt
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
changes in activity during germination, distribution between cotyledons and roots
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
the intracellular localization is changed depending on the growth phase. Distributed in the cytosol until the initial logarithmic phase (12 h), then delivered to an organelle with a granule structure in the middle logarithmic phase (14 h), and finally localized at the vacuole membranes in the stationary phase (18-20 h)
brenda
-
the intracellular localization is changed depending on the growth phase. Distributed in the cytosol until the initial logarithmic phase (12 h), then delivered to an organelle with a granule structure in the middle logarithmic phase (14 h), and finally localized at the vacuole membranes in the stationary phase (18-20 h)
-
brenda
the intracellular localization is changed depending on the growth phase. Distributed in the cytosol until the initial logarithmic phase (12 h), then delivered to an organelle with a granule structure in the middle logarithmic phase (14 h), and finally localized at the vacuole membranes in the stationary phase (18-20 h)
brenda
-
the intracellular localization is changed depending on the growth phase. Distributed in the cytosol until the initial logarithmic phase (12 h), then delivered to an organelle with a granule structure in the middle logarithmic phase (14 h), and finally localized at the vacuole membranes in the stationary phase (18-20 h)
-
brenda
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
malfunction
the disruption of EGH1 in Saccharomyces cerevisiae BY4741 (egh1DELTA) results in the accumulation of ergosteryl 3-O-beta-glucoside and fragmentation of vacuoles
malfunction
disruption of the EGCrP2 gene (egcrp2) results in the accumulation of ergosteryl 3-O-beta-glucoside, a major sterylglucoside in fungi. Distinct growth arrest, a dysfunction in cell budding, and an abnormal vacuole morphology are detected in the egcrp2-disrupted mutants
malfunction
-
disruption of the EGCrP2 gene (egcrp2) results in the accumulation of ergosteryl 3-O-beta-glucoside, a major sterylglucoside in fungi. Distinct growth arrest, a dysfunction in cell budding, and an abnormal vacuole morphology are detected in the egcrp2-disrupted mutants
-
malfunction
-
the disruption of EGH1 in Saccharomyces cerevisiae BY4741 (egh1DELTA) results in the accumulation of ergosteryl 3-O-beta-glucoside and fragmentation of vacuoles
-
physiological function
involved in sterylglucoside catabolism and vacuole formation
physiological function
the enzyme is involved in the catabolism of ergosteryl 3-O-beta-glucoside in the vacuoles of Cryptococcus neoformans
physiological function
-
the enzyme is involved in the catabolism of ergosteryl 3-O-beta-glucoside in the vacuoles of Cryptococcus neoformans
-
physiological function
-
involved in sterylglucoside catabolism and vacuole formation
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GBA2_HUMAN
927
0
104649
Swiss-Prot
GBA2_MOUSE
918
0
103294
Swiss-Prot
GBA2_RAT
912
0
102747
Swiss-Prot
GLCM_BOVIN
536
0
59855
Swiss-Prot
GLCM_HUMAN
536
0
59716
Swiss-Prot
GLCM_MOUSE
515
0
57622
Swiss-Prot
GLCM_PANTR
536
0
59664
Swiss-Prot
GLCM_PIG
536
0
59874
Swiss-Prot
GLCM_PONAB
536
0
59682
Swiss-Prot
A0A228BX09_CRYNV
851
0
94743
TrEMBL
EGH1_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
764
0
86979
Swiss-Prot
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
x * 120000, SDS-PAGE
?
-
x * 120000, SDS-PAGE
-
?
-
x * 138000, SDS-PAGE
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli BL21 (DE3)
expressed in Escherichia coli BL21 (DE3)
expressed in Escherichia coli BL21 (DE3)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pharmacology
the enzyme may be a promising target for anti-cryptococcal drugs
pharmacology
-
the enzyme may be a promising target for anti-cryptococcal drugs
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Kalinowska, M.; Wojciechowski, Z.A.
Purification and some properties of steryl beta-D-glucoside hydrolase from Sinapis alba seedlings
Phytochemistry
17
1533-1537
1978
Sinapis alba
-
brenda
Kalinowska, M.; Wojciechowski, Z.A.
Modulation of activities of steryl glucoside hydrolase and UDPG:sterol glucosyltransferase from Sinapis alba by detergents and lipids
Phytochemistry
25
45-49
1986
Sinapis alba
-
brenda
Kalinowska, M.; Wojciechowski, Z.A.
Sterol conjugate interconversions during germination of white mustard (Sinapis alba)
Phytochemistry
23
2485-2488
1984
Sinapis alba
-
brenda
Watanabe, T.; Tani, M.; Ishibashi, Y.; Endo, I.; Okino, N.; Ito, M.
Ergosteryl-beta-glucosidase (Egh1) involved in sterylglucoside catabolism and vacuole formation in Saccharomyces cerevisiae
Glycobiology
25
1079-1089
2015
Saccharomyces cerevisiae (P40566), Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508 (P40566)
brenda
Watanabe, T.; Ito, T.; Goda, H.; Ishibashi, Y.; Miyamoto, T.; Ikeda, K.; Taguchi, R.; Okino, N.; Ito, M.
Sterylglucoside catabolism in cryptococcus neoformans with endoglycoceramidase-related protein 2 (EGCrP2), the first steryl-beta-glucosidase identified in fungi
J. Biol. Chem.
290
1005-1019
2015
Cryptococcus neoformans (A0A228BX09), Cryptococcus neoformans, Cryptococcus neoformans ATCC 208821 (A0A228BX09)
brenda
Select items on the left to see more content.
html completed