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Literature summary for 3.2.1.100 extracted from

  • Domingues, M.N.; Souza, F.H.M.; Vieira, P.S.; de Morais, M.A.B.; Zanphorlin, L.M.; Dos Santos, C.R.; Pirolla, R.A.S.; Honorato, R.V.; de Oliveira, P.S.L.; Gozzo, F.C.; Murakami, M.T.
    Structural basis of exo-beta-mannanase activity in the GH2 family (2018), J. Biol. Chem., 293, 13636-13649 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.100

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21(DE3) Xanthomonas citri

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor-diffusion method at 18°C Xanthomonas citri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2
-
 beta-mannan pH 5.5, 40°C Xanthomonas citri
5.11
-
 mannohexaose pH 5.5, 40°C Xanthomonas citri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
99480
-
 calculation from sequence Xanthomonas citri

Organism

Organism UniProt Comment Textmining
Xanthomonas citri Q8PI23
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Xanthomonas citri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-mannopyranoside + H2O
-
 Xanthomonas citri 4-nitrophenol + beta-D-mannopyranoside
-
 ?
beta-mannan + H2O
-
 Xanthomonas citri D-mannose + ?
-
 ?
galactomannan + H2O
-
 Xanthomonas citri D-mannose + ?
-
 ?
mannobiose + H2O increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides Xanthomonas citri mannose +
-
 ?
mannohexaose + H2O increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides Xanthomonas citri D-mannose + ?
-
 ?
mannopentaose + H2O increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides Xanthomonas citri D-mannose + ?
-
 ?
mannotetraose + H2O increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides Xanthomonas citri D-mannose + ?
-
 ?
mannotriose + H2O increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides Xanthomonas citri mannobiose + D-mannose
-
 ?

Synonyms

Synonyms Comment Organism
exo-beta-mannanase
-
 Xanthomonas citri
XacMan2A
-
 Xanthomonas citri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Xanthomonas citri

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 40% of maximal activity, 50°C: about 40% of maximal activity Xanthomonas citri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 4 h, about 50% loss of activity Xanthomonas citri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.53
-
 beta-mannan pH 5.5, 40°C Xanthomonas citri
126.86
-
 mannohexaose pH 5.5, 40°C Xanthomonas citri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Xanthomonas citri

pH Range

pH Minimum pH Maximum Comment Organism
5 7 pH 5.0: about 70% of maximal activity, pH 7.0: about 45% of maximal activity Xanthomonas citri

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.98
-
 beta-mannan pH 5.5, 40°C Xanthomonas citri
24.82
-
 mannohexaose pH 5.5, 40°C Xanthomonas citri