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3.2.1.100
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flavefaciens
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acid-swollen
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chrysosporium
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pulverulentum
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stercorarium
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avicel
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avicelase
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sporotrichum
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cellulolytic
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ruminococcus
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lignorum
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
xacman2a, exo-beta-mannanase,
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exo-1,4-beta-mannobiohydrolase
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mannan 1,4-beta-mannobiosidase
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mannobiohydrolase, exo-1,4-beta-
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exo-beta-mannanase
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(1,4-beta-D-mannopyranosyl)n-1,4-beta-D-mannopyranose + H2O = (1,4-beta-D-mannopyranosyl)n-2-1,4-beta-D-mannopyranose + 4-O-beta-D-mannopyranosyl-beta-D-mannopyranose
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hydrolysis of O-glycosyl bond
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4-beta-D-mannan mannobiohydrolase
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4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannopyranoside
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beta-mannan + H2O
D-mannose + ?
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codium mannan + H2O
mannobiose
coffee mannan + H2O
mannobiose
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sole product
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galactomannan + H2O
D-mannose + ?
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mannobiose + H2O
mannose +
increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides
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mannobiosylmannitol + H2O
mannobiose + mannitol
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mannohexaose + H2O
D-mannose + ?
increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides
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mannopentaose + H2O
D-mannose + ?
increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides
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mannopentaose + H2O
mannobiose + mannose
mannotetraose
mannohexaose
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transglycosylation
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mannotetraose + H2O
D-mannose + ?
increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides
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mannotetraose + H2O
mannobiose
mannotetraosylmannitol + H2O
mannobiose + mannosylmannitol + ?
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mannotriose + H2O
mannobiose + D-mannose
increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides
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mannotriose + H2O
mannobiose + mannose
mannotriose + mannose
mannopentaose
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transglycosylation
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additional information
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codium mannan + H2O
mannobiose
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sole product
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codium mannan + H2O
mannobiose
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sole product
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mannopentaose + H2O
mannobiose + mannose
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mannopentaose + H2O
mannobiose + mannose
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mannotetraose + H2O
mannobiose
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mannotetraose + H2O
mannobiose
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mannotriose + H2O
mannobiose + mannose
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mannotriose + H2O
mannobiose + mannose
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additional information
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not: mannobiose, p-nitrophenyl-beta-D-mannoside, konjac glucomannan, guar gum galactomannan
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additional information
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not: mannobiose, p-nitrophenyl-beta-D-mannoside, konjac glucomannan, guar gum galactomannan
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Ag+
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1 mM, complete inhibition
Cu2+
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1 mM, complete inhibition
EDTA
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1 mM, 30-50% inhibition
Fe3+
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1 mM, 30-50% inhibition
Hg2+
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1 mM, complete inhibition
p-chloromercuribenzoate
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1 mM, 30-50% inhibition
Pb2+
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1 mM, 30-50% inhibition
Zn2+
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1 mM, 30-50% inhibition
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4.2
beta-mannan
pH 5.5, 40°C
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5.11
mannohexaose
pH 5.5, 40°C
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12.53
beta-mannan
pH 5.5, 40°C
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126.86
mannohexaose
pH 5.5, 40°C
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2.98
beta-mannan
pH 5.5, 40°C
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24.82
mannohexaose
pH 5.5, 40°C
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5 - 7
pH 5.0: about 70% of maximal activity, pH 7.0: about 45% of maximal activity
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20 - 50
20°C: about 40% of maximal activity, 50°C: about 40% of maximal activity
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ssp. anaerogenes
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brenda
ssp. anaerogenes
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brenda
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UniProt
brenda
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brenda
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brenda
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brenda
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brenda
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Q8PI23_XANAC
Xanthomonas axonopodis pv. citri (strain 306)
896
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100375
TrEMBL
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99480
calculation from sequence
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hanging-drop vapor-diffusion method at 18°C
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5 - 8.5
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20°C, 20 h
135706
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40
4 h, about 50% loss of activity
45
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15 min, stable below
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15 min, complete loss of activity
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overexpression in Escherichia coli BL21(DE3)
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Araki, T.; Kitamikado, M.
Purification and characterization of a novel exo-beta-mannanase from Aeromonas sp. F-25
J. Biochem.
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1181-1186
1982
Aeromonas hydrophila, Aeromonas hydrophila F-25
brenda
Domingues, M.N.; Souza, F.H.M.; Vieira, P.S.; de Morais, M.A.B.; Zanphorlin, L.M.; Dos Santos, C.R.; Pirolla, R.A.S.; Honorato, R.V.; de Oliveira, P.S.L.; Gozzo, F.C.; Murakami, M.T.
Structural basis of exo-beta-mannanase activity in the GH2 family
J. Biol. Chem.
293
13636-13649
2018
Xanthomonas citri (Q8PI23), Xanthomonas citri
brenda
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3.2.1.100 mannan 1,4-mannobiosidase
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