Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.13.2.1 extracted from

  • Hao, W.; Li, Y.; Shan, Q.; Han, T.; Li, W.; He, S.; Zhu, K.; Li, Y.; Tan, X.; Gu, J.
    Characterization of human S-adenosyl-homocysteine hydrolase in vitro and identification of its potential inhibitors (2017), J. Enzyme Inhib. Med. Chem., 32, 1209-1215 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.13.2.1

Application

Application Comment Organism
drug development because of the prominent role of SAM-dependent transmethylation in capped methylated structure production at the 5'-terminus of viral mRNA, SAHH inhibitors could be developed to be broad-spectrum antiviral agents Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene sahH, codon-optimized gene, recombinant expression of C-terminally His6-tagged enzyme in Pichia pastoris under control of the strong AOX1 promoter Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(1R,2S,3R)-3-(4-amino-7-fluoro-1H-imidazo[4,5-c]pyridin-1-yl)cyclopentane-1,2-diol CAS:1214735-71-3 Homo sapiens
(2E)-3-(4-chlorophenyl)prop-2-enoic acid
-
 Homo sapiens
(2E)-3-(4-methoxyphenyl)prop-2-enoic acid
-
 Homo sapiens
1-(bromomethyl)-2,4-difluorobenzene
-
 Homo sapiens
1H-benzotriazol-1-ol
-
 Homo sapiens
4-(3-hydroxyprop-1-en-1-yl)-2-methoxyphenol coniferyl alcohol, molecular docking studies show that coniferyl alcohol is well docked into the active cavity of SAHH. Several H-bonds are formed between them, which stabilize coniferyl alcohol in the active site of rSAHH with a proper conformation Homo sapiens
4-chloro-1,3-dihydroxyphenol
-
 Homo sapiens
4-chlorobenzene-1-carboperoxoic acid
-
 Homo sapiens
4-fluorobenzoic acid
-
 Homo sapiens
4-methoxyaniline
-
 Homo sapiens
4-methoxybenzaldehyde i.e. anisaldehyde Homo sapiens
4-[(1E)-3-hydroxyprop-1-en-1-yl]-2-methoxyphenol
-
 Homo sapiens
ethyl 4-aminobenzoate
-
 Homo sapiens
methyl (2E)-3-[4-(bromomethyl)phenyl]prop-2-enoate
-
 Homo sapiens
additional information molecular docking study and simulations Homo sapiens
neplanocin A NepA, a natural product that is cytotoxic upon long term exposure and shows adenosine deaminase and adenosine kinase activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics, double-reciprocal form of the Michaelis-Menten equation Homo sapiens
0.0218
-
 S-adenosyl-L-homocysteine pH 6.5, 41°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-homocysteine + H2O Homo sapiens
-
 L-homocysteine + adenosine
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens P23526
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Pichia pastoris by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-homocysteine + H2O
-
 Homo sapiens L-homocysteine + adenosine
-
 r

Subunits

Subunits Comment Organism
? x * 47500, recombinant His-tagged enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
S-adenosyl-homocysteine hydrolase
-
 Homo sapiens
SAHH
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
41
-
 recombinant His-tagged enzyme Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 49 activity range, profile overview Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 recombinant His-tagged enzyme Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4.5 10.5 activity range, profile overview Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000015
-
 pH 6.5, 41°C, recombinant enzyme Homo sapiens neplanocin A
0.000034
-
 pH 6.5, 41°C, recombinant enzyme Homo sapiens 4-[(1E)-3-hydroxyprop-1-en-1-yl]-2-methoxyphenol
0.00004
-
 pH 6.5, 41°C, recombinant enzyme Homo sapiens (1R,2S,3R)-3-(4-amino-7-fluoro-1H-imidazo[4,5-c]pyridin-1-yl)cyclopentane-1,2-diol
0.000565
-
 pH 6.5, 41°C, recombinant enzyme Homo sapiens 4-chlorobenzene-1-carboperoxoic acid
0.000571
-
 pH 6.5, 41°C, recombinant enzyme Homo sapiens (2E)-3-(4-chlorophenyl)prop-2-enoic acid
0.000679
-
 pH 6.5, 41°C, recombinant enzyme Homo sapiens 4-fluorobenzoic acid
0.000814
-
 pH 6.5, 41°C, recombinant enzyme Homo sapiens methyl (2E)-3-[4-(bromomethyl)phenyl]prop-2-enoate
0.000899
-
 pH 6.5, 41°C, recombinant enzyme Homo sapiens 4-methoxyaniline
0.000913
-
 pH 6.5, 41°C, recombinant enzyme Homo sapiens 1-(bromomethyl)-2,4-difluorobenzene
0.000986
-
 pH 6.5, 41°C, recombinant enzyme Homo sapiens (2E)-3-(4-methoxyphenyl)prop-2-enoic acid
0.00111
-
 pH 6.5, 41°C, recombinant enzyme Homo sapiens 4-chloro-1,3-dihydroxyphenol
0.001245
-
 pH 6.5, 41°C, recombinant enzyme Homo sapiens 1H-benzotriazol-1-ol
0.001399
-
 pH 6.5, 41°C, recombinant enzyme Homo sapiens ethyl 4-aminobenzoate
0.002131
-
 pH 6.5, 41°C, recombinant enzyme Homo sapiens 4-methoxybenzaldehyde

General Information

General Information Comment Organism
malfunction the inhibition of SAHH can lead to intracellular SAH accumulation, which triggers the negative feedback inhibition to suppress the S-adenosyl-L-methionine (SAM)-dependent transmethylation Homo sapiens
metabolism the enzyme is involved in the activated methyl cycle (AMC) of mammalian cells, overview Homo sapiens
physiological function SAHH plays a critical role in the mammalian methylation processes Homo sapiens