Literature summary for 3.13.2.1 extracted from

Hsu, J.F.; Chuang, W.J.; Shiesh, S.C.; Lin, Y.S.; Liu, C.C.; Wang, C.C.; Fu, T.F.; Tsai, J.H.; Tsai, W.L.; Huang, Y.J.; Hsieh, Y.H.; Wu, J.J.; Lin, M.T.; Huang, W.
Streptococcal pyrogenic exotoxin B cleaves human S-adenosylhomocysteine hydrolase and induces hypermethioninemia (2008), J. Infect. Dis., 198, 367-374.

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Application

Application	Comment	Organism
molecular biology	Streptococcal pyrogenic exotoxin B cleaves human S-adenosylhomocysteine hydrolase and induces hypermethioninemia	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	using a yeast two-hybrid system it shown that AdoHcyase directly interacts with Streptococcal pyrogenic exotoxin B protease. Furthermore, it is shown that AdoHcyase gets cleaved and inactivated by Streptococcal pyrogenic exotoxin B protease, inducing hypermethioninemia	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography and gel filtration	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
fibroblast cell line	human kidney fibroblast cell line 293T cells: AdoHcyase protein levels and enzymatic activities are significantly higher in human cells infected with the Streptococcus pyogenes SW510 speB mutant strain than in cells infected with the NZ131 wild-type strain	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	using a yeast two-hybrid system it shown that AdoHcyase directly interacts with Streptococcal pyrogenic exotoxin B protease. Furthermore, it is shown that AdoHcyase gets cleaved and inactivated by Streptococcal pyrogenic exotoxin B protease, inducing hypermethioninemia	Homo sapiens	?	-	?
S-adenosyl-L-homocysteine + H2O	-	Homo sapiens	adenosine + L-homocysteine	-	?

Synonyms

Synonyms	Comment	Organism
AdoHcyase	-	Homo sapiens
S-adenosylhomocysteine hydrolase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Homo sapiens

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Release 2023.1 (January 2023)