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Literature summary for 3.13.2.1 extracted from
- Adenosylhomocysteinase from yellow lupin seeds. Purification and properties (1977), Eur. J. Biochem., 80, 517-523.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| adenosine deaminase | - |
Lupinus luteus | |
| adenosine nucleosidase | - |
Lupinus luteus |
General Stability
| General Stability | Organism |
|---|---|
| purified enzyme tolerates repeated freeze-thawing | Lupinus luteus |
| tendency of purified enzyme to form multimers | Lupinus luteus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-ethylmaleimide | - |
Lupinus luteus |  |
| p-hydroxymercuribenzoate | - |
Lupinus luteus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0023 | - |
adenosine | - |
Lupinus luteus | |
| 0.012 | - |
S-adenosyl-L-homocysteine | - |
Lupinus luteus | |
| 4.6 | - |
DL-homocysteine | - |
Lupinus luteus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS-PAGE | Lupinus luteus |
| 110000 | - |
gel filtration | Lupinus luteus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-homocysteine + H2O | Lupinus luteus | - |
adenosine + L-homocysteine | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lupinus luteus | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| 2-mercaptoethanol essential for enzymatic activity, in absence 50% loss of activity | Lupinus luteus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1193.5fold to homogeneity | Lupinus luteus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine | reaction equilibrium is in vitro strongly in direction of synthesis of S-adenosyl-L-homocysteine | Lupinus luteus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Lupinus luteus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.7 | 4.7 | - |
Lupinus luteus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10°C, loss of 50% of activity after 1 month | Lupinus luteus |
| -20°C, stable for at least 2 months | Lupinus luteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-deaza-8-azaadenosine + homocysteine | formycin A | Lupinus luteus | ? | - |
r | |
| adenosine N1-oxide + homocysteine | - |
Lupinus luteus | ? | - |
r | |
| carboxylic analogue of adenosine | - |
Lupinus luteus | ? | - |
r | |
| DL-homocysteine + adenosine | - |
Lupinus luteus | S-adenosyl-DL-homocysteine | - |
r | |
| nebularine + homocysteine | - |
Lupinus luteus | ? | - |
r | |
| S-adenosyl-L-homocysteine + H2O | - |
Lupinus luteus | adenosine + L-homocysteine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 55000, pore gradient gel electrophoresis | Lupinus luteus |
| monomer | 1 * 55000, pore gradient gel electrophoresis, only dimeric form is enzymatically active | Lupinus luteus |
| tetramer | 4 * 55000, pore gradient gel electrophoresis | Lupinus luteus |
| trimer | 3 * 55000, pore gradient gel electrophoresis | Lupinus luteus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Lupinus luteus |
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