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Literature summary for 3.13.2.1 extracted from

  • Hershfield, M.S.; Aiyar, V.N.; Premakumar, R.; Small, W.C.
    S-Adenosylhomocysteine hydrolase from human placenta. Affinity purification and characterization (1985), Biochem. J., 230, 43-52.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.13.2.1

Inhibitors

Inhibitors Comment Organism Structure
4',5'-dehydroadenosine
-
 Homo sapiens
adenine arabinoside
-
 Homo sapiens
adenosine
-
 Homo sapiens
dithiothreitol together with adenosine Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
 adenosine
-
 Homo sapiens
0.0076
-
 S-adenosyl-L-homocysteine
-
 Homo sapiens
0.11
-
 4',5'-dehydroadenosine
-
 Homo sapiens
0.2
-
 L-homocysteine
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 4 * 47000-48000, SDS-PAGE, 4 * 49000, amino acid determination Homo sapiens
189000
-
 analytical ultrafiltration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-homocysteine + H2O Homo sapiens
-
 adenosine + L-homocysteine
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2200fold to homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
CCRF-CEM cell
-
 Homo sapiens
-
placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activity is correlated with enzyme-bound NAD+/NADH ratio Homo sapiens
4.4
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-70°C, 1-2 mg enzyme per ml, loss of less than 10% of activity over 6 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4',5'-dehydroadenosine + H2O favors formation of 4',5'-dehydroadenosine, precursor reaction Homo sapiens adenosine
-
 r
S-adenosyl-L-homocysteine + H2O
-
 Homo sapiens adenosine + L-homocysteine
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 47000-48000, SDS-PAGE, 4 * 49000, amino acid determination Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 97% of activity is lost Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens