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Literature summary for 3.13.2.1 extracted from
- Adenosylhomocysteinase (bovine) (1987), Methods Enzymol., 143, 377-383.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (+/-)-aristeromycin | carbocyclic adenosine; competitive | Bos taurus |  |
| 2'-deoxyadenosine | irreversible | Bos taurus | |
| 2-aza-3-deazaadenosine | competitive | Bos taurus | |
| 2-chloro-3-deazaadenosine | irreversible | Bos taurus | |
| 2-chloroadenosine | irreversible | Bos taurus | |
| 3-deaza-(+/-)-aristeromycin | carbocyclic 3-deazaadenosine; competitive | Bos taurus | |
| 3-deazaadenosine | competitive | Bos taurus | |
| 7-deaza-8-azaadenosine | competitive; formycin A | Bos taurus | |
| 8-Aminoadenosine | competitive | Bos taurus | |
| 8-azaadenosine | competitive | Bos taurus | |
| 9-beta-D-arabinofuranosyl-2-chloroadenine | irreversible | Bos taurus | |
| 9-beta-D-arabinofuranosyl-2-fluoroadenine | irreversible | Bos taurus | |
| 9-beta-D-arabinofuranosyl-3-deazaadenine | irreversible | Bos taurus | |
| 9-beta-D-arabinofuranosyladenine | irreversible | Bos taurus | |
| adenosine N1-oxide | competitive | Bos taurus | |
| Inosine | competitive | Bos taurus | |
| N6-methyladenosine | competitive | Bos taurus | |
| nebularine | competitive | Bos taurus | |
| Nucleocidin | irreversible | Bos taurus | |
| Pyrazomycin | competitive | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0019 | - |
adenosine | - |
Bos taurus | |
| 0.15 | - |
L-homocysteine | - |
Bos taurus | |
| 2 | - |
Inosine | - |
Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-homocysteine + H2O | Bos taurus | - |
adenosine + L-homocysteine | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine | reaction equilibrium is in vitro strongly in direction of synthesis of S-adenosyl-L-homocysteine | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.5 | 0.65 | - |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, lyophilized, purified enzyme | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (+/-)-aristeromycin + homocysteine | carbocyclic adenosine | Bos taurus | ? | - |
r | |
| 2-aza-3-deazaadenosine + homocysteine | - |
Bos taurus | ? | - |
r | |
| 3-deaza-(+/-)-aristeromycin + homocysteine | carbocyclic 3-deazaadenosine | Bos taurus | ? | - |
r | |
| 3-deazaadenosine + L-homocysteine | - |
Bos taurus | 3-deazaadenosylhomocysteine | - |
r | |
| 7-deaza-8-azaadenosine + homocysteine | formycin A | Bos taurus | ? | - |
r | |
| 8-aminoadenosine + homocysteine | - |
Bos taurus | ? | - |
r | |
| 8-azaadenosine + homocysteine | - |
Bos taurus | ? | - |
r | |
| adenosine N1-oxide + homocysteine | - |
Bos taurus | ? | - |
r | |
| DL-homocysteine + adenosine | - |
Bos taurus | S-adenosyl-DL-homocysteine | - |
r | |
| inosine + homocysteine | - |
Bos taurus | ? | - |
r | |
| N6-methyladenosine + homocysteine | - |
Bos taurus | ? | - |
r | |
| nebularine + homocysteine | - |
Bos taurus | ? | - |
r | |
| pyrazomycin + homocysteine | - |
Bos taurus | ? | - |
r | |
| S-adenosyl-L-homocysteine + H2O | - |
Bos taurus | adenosine + L-homocysteine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 5000 | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Bos taurus | |
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