Cloned (Comment) | Organism |
---|---|
recombinant expression of the enzyme in Escherichia coli BL21(DE3)pLysS, subcloning in Escherichia coli strain DH5alpha. To achieve the extracellular secretion of enzyme OPH in Escherichia coli, the complete opd gene along with its native signal peptide is codon-optimized and expressed in Escherichia coli BL21(DE3)pLysS. The secretion of OPH into the extracellular medium is highly affected by culture conditions, e.g. Co2+ decreases the secretion of the enzyme, while IPTG causes an enhancement in extracellular OPH secretion | Flavobacterium sp. MTCC 2495 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the premature enzyme contains a signal peptide | Flavobacterium sp. MTCC 2495 | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | required | Flavobacterium sp. MTCC 2495 | |
additional information | divalent cations are essential for activity | Flavobacterium sp. MTCC 2495 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. MTCC 2495 | Q5UB52 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the premature enzyme contains a signal peptide that is cleaved off during maturation | Flavobacterium sp. MTCC 2495 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
paraoxon + H2O | - |
Flavobacterium sp. MTCC 2495 | diethyl phosphate + 4-nitrophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, premature enzyme with signal peptide, SDS-PAGE, x * 37000, mature enzyme, SDS-PAGE | Flavobacterium sp. MTCC 2495 |
Synonyms | Comment | Organism |
---|---|---|
opd | - |
Flavobacterium sp. MTCC 2495 |
OPH | - |
Flavobacterium sp. MTCC 2495 |
organophosphorus hydrolase | - |
Flavobacterium sp. MTCC 2495 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Flavobacterium sp. MTCC 2495 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
assay at | Flavobacterium sp. MTCC 2495 |