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Literature summary for 3.1.8.1 extracted from
- Novel purification strategy for human PON1 and inhibition of the activity by cephalosporin and aminoglikozide derived antibiotics (2006), Biochimie, 88, 565-574.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cefazolin | inhibits the serum and liver PON1, competitive inhibition | Homo sapiens |  |
| gentamycin sulfate | inhibits the serum and liver PON1, noncompetitive inhibition | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.16 | - |
paraoxon | pH 8.0, 37°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lipid particle | the enzyme is associated to HDL | Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | dependent on | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 227fold by ammonium sulfate fractionation and L-tyrosine-1-naphthylamine hydrophobic interaction chromatography to homogeneity | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Hep-G2 cell | - |
Homo sapiens | - |
| hepatoma cell | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| serum | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1730 | - |
purified serum enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| paraoxon + H2O | - |
Homo sapiens | diethylphosphate + 4-nitrophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| paraoxonase | - |
Homo sapiens |
| PON1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | in vivo and in vitro inhibition kinetic analysis, overview | Homo sapiens | |
| 0.0012 | - |
cefazolin | pH 8.0, 37°C, serum PON1, versus paraoxon | Homo sapiens | |
| 0.026 | - |
gentamycin sulfate | pH 8.0, 37°C, serum PON1 | Homo sapiens | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| additional information | - |
- |
Homo sapiens | additional information | |
| 0.0084 | - |
pH 8.0, 37°C, serum PON1 | Homo sapiens | cefazolin | |
| 0.887 | - |
pH 8.0, 37°C, serum PON1 | Homo sapiens | gentamycin sulfate | |
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