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Literature summary for 3.1.8.1 extracted from
- Altering the substrate specificity of organophosphorus hydrolase for enhanced hydrolysis of chlorpyrifos (2004), Appl. Environ. Microbiol., 70, 4681-4685.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| wild-type and variants of gene opd, DNA and amino acid sequence determination of gene variants, overview, expression of OPHs at the cell surface of strain XL1-Blue using plasmid pINCOP | Escherichia coli K-12 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A14T/A80V/K185R | naturally occurring OPH genetic variant B1368, the variant shows altered kinetics with chlorpyrifos compared to the wild-type enzyme | Escherichia coli K-12 |
| A14T/A80V/K185R/H254R/I274N | naturally occurring OPH genetic variant B2136, the variant shows altered kinetics with chlorpyrifos compared to the wild-type enzyme | Escherichia coli K-12 |
| A14T/A80V/K185R/H257Y/I274N | naturally occurring OPH genetic variant 22A11, the variant shows altered kinetics with chlorpyrifos compared to the wild-type enzyme | Escherichia coli K-12 |
| A14T/A80V/K185R/I274N | naturally occurring OPH genetic variant A1030, the variant shows altered kinetics with chlorpyrifos compared to the wild-type enzyme | Escherichia coli K-12 |
| A14T/L17P/A80V/V116I/K185R/A203T/I274N/P342S | naturally occurring OPH genetic variant B3561, the variant shows altered kinetics with chlorpyrifos compared to the wild-type enzyme | Escherichia coli K-12 |
| A14T/R67H/A80V/L87S/Q148R/K185R | naturally occurring OPH genetic variant A1467, the variant shows altered kinetics with chlorpyrifos compared to the wild-type enzyme | Escherichia coli K-12 |
| additional information | analysis of stucture, and activity and kinetics with chlorpyrifos of genetic variants, 22A11, A1030, A1467, B1368, B2136, and B3561, overview | Escherichia coli K-12 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.032 | - |
chlorpyrifos | pH 8.0, 37°C, genetic variant B2136 | Escherichia coli K-12 |  |
| 0.14 | - |
chlorpyrifos | pH 8.0, 37°C, genetic variant B3561 | Escherichia coli K-12 | |
| 0.19 | - |
chlorpyrifos | pH 8.0, 37°C, genetic variant 22A11 | Escherichia coli K-12 | |
| 0.22 | - |
chlorpyrifos | pH 8.0, 37°C, genetic variant B1368 | Escherichia coli K-12 | |
| 0.26 | - |
chlorpyrifos | pH 8.0, 37°C, wild-type enzyme | Escherichia coli K-12 | |
| 0.27 | - |
chlorpyrifos | pH 8.0, 37°C, genetic variant A1467 | Escherichia coli K-12 | |
| 0.51 | - |
chlorpyrifos | pH 8.0, 37°C, genetic variant A1030 | Escherichia coli K-12 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Escherichia coli K-12 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli K-12 | P37669 | gene yiaH, variant B3561; wild-type and variants of gene opd, e.g. variant B3561 | - |
| Escherichia coli K-12 XL1-Blue | P37669 | gene yiaH, variant B3561; wild-type and variants of gene opd, e.g. variant B3561 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and genetic variants | Escherichia coli K-12 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli K-12 |
| 0.1 | - |
about, wild-type enzyme, substrate chlorpyrifos | Escherichia coli K-12 |
| 1 | - |
about, genetic variants A1030 and A1467, substrate chlorpyrifos | Escherichia coli K-12 |
| 5 | - |
about, genetic variant B2136, substrate chlorpyrifos | Escherichia coli K-12 |
| 27 | - |
about, genetic variant B1368, substrate chlorpyrifos | Escherichia coli K-12 |
| 35 | - |
about, genetic variant B3561, substrate chlorpyrifos | Escherichia coli K-12 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorpyrifos + H2O | a popular pesticide used in agricultural crop protection causes widespread contamination, degradation by evolutionary improved genetic OPH variants, e.g. variants B3561 and B1368, overview | Escherichia coli K-12 | 3,5,6-trichloro-pyridin-2-ol + diethyl thiophosphate | - |
? | |
| chlorpyrifos + H2O | the wild-type enzyme shows 1000fold lower activity compared to substrate paraoxon, analysis of activity with chlorpyrifos of genetic variants, 22A11, A1030, A1467, B1368, B2136, and B3561, the wild-type shows extremely low activity while variants B3561 and B1368 show high activity, overview | Escherichia coli K-12 | 3,5,6-trichloro-pyridin-2-ol + diethyl thiophosphate | - |
? | |
| chlorpyrifos + H2O | a popular pesticide used in agricultural crop protection causes widespread contamination, degradation by evolutionary improved genetic OPH variants, e.g. variants B3561 and B1368, overview | Escherichia coli K-12 XL1-Blue | 3,5,6-trichloro-pyridin-2-ol + diethyl thiophosphate | - |
? | |
| chlorpyrifos + H2O | the wild-type enzyme shows 1000fold lower activity compared to substrate paraoxon, analysis of activity with chlorpyrifos of genetic variants, 22A11, A1030, A1467, B1368, B2136, and B3561, the wild-type shows extremely low activity while variants B3561 and B1368 show high activity, overview | Escherichia coli K-12 XL1-Blue | 3,5,6-trichloro-pyridin-2-ol + diethyl thiophosphate | - |
? | |
| coumaphos + H2O | - |
Escherichia coli K-12 | 3-chloro-4-methylumbelliferone + diethyl thiophosphate | - |
? | |
| methyl parathion + H2O | - |
Escherichia coli K-12 | 4-nitrophenol + dimethyl thiophosphate | - |
? | |
| methyl parathion + H2O | - |
Escherichia coli K-12 XL1-Blue | 4-nitrophenol + dimethyl thiophosphate | - |
? | |
| additional information | substrate specificity of wild-type and genetic variants, overview | Escherichia coli K-12 | ? | - |
? | |
| additional information | substrate specificity of wild-type and genetic variants, overview | Escherichia coli K-12 XL1-Blue | ? | - |
? | |
| paraoxon + H2O | - |
Escherichia coli K-12 | 4-nitrophenol + diethyl phosphate | - |
? | |
| paraoxon + H2O | preferred substrate | Escherichia coli K-12 | 4-nitrophenol + diethyl phosphate | - |
? | |
| paraoxon + H2O | - |
Escherichia coli K-12 XL1-Blue | 4-nitrophenol + diethyl phosphate | - |
? | |
| parathion + H2O | - |
Escherichia coli K-12 | 4-nitrophenol + diethyl thiophosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| inner membrane protein YiaH | - |
Escherichia coli K-12 |
| OPH | - |
Escherichia coli K-12 |
| organophosphorus hydrolase | - |
Escherichia coli K-12 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli K-12 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.2 | - |
chlorpyrifos | pH 8.0, 37°C, wild-type enzyme | Escherichia coli K-12 | |
| 73.8 | - |
chlorpyrifos | pH 8.0, 37°C, wild-type enzyme | Escherichia coli K-12 | |
| 1430 | - |
chlorpyrifos | pH 8.0, 37°C, genetic variant A1467 | Escherichia coli K-12 | |
| 2610 | - |
chlorpyrifos | pH 8.0, 37°C, genetic variant A1030 | Escherichia coli K-12 | |
| 3540 | - |
chlorpyrifos | pH 8.0, 37°C, genetic variant B2136 | Escherichia coli K-12 | |
| 20100 | - |
chlorpyrifos | pH 8.0, 37°C, genetic variant 22A11 | Escherichia coli K-12 | |
| 21500 | - |
chlorpyrifos | pH 8.0, 37°C, genetic variant B1368 | Escherichia coli K-12 | |
| 30900 | - |
chlorpyrifos | pH 8.0, 37°C, genetic variant B3561 | Escherichia coli K-12 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | assay at | Escherichia coli K-12 |
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