Activating Compound | Comment | Organism | Structure |
---|---|---|---|
furin | UCE is synthesized as an inactive proenzyme that is specifically activated by the endoprotease furin | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in mouse L-cells, in CHO-K1 cells and in Sf9 insect cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y488A | mutation in the 488YHPL internalization signal present in the cytoplasmic tail, mutant UCE accumulates on the cell surface | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi membrane | mainly localized to the trans-Golgi network, cycles between the network and the plasma membrane, type I transmembrane glycoprotein, containing a 24-amino acid signal sequence, a luminal domain of 423 residues, a 27-residue transmembrane region and a 41-residue cytoplasmic tail | Homo sapiens | 139 | - |
plasma membrane | mainly localized to the trans-Golgi network and cycles between the network and plasma membrane | Homo sapiens | 5886 | - |
soluble | recombinant UCE expressed in CHO-K1 cells and in Sf9 insect cells | Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O | Homo sapiens | removes a covering N-acetylglucosamine from the mannose 6-phosphate recognition marker on lysosomal acid hydrolases | N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
proteolytic modification | UCE is synthesized as an inactive proenzyme that is activated by the endoprotease furin, which is localized in the trans-Golgi network and cleaves an RARLPR/D sequence to release a 24-amino acid propiece | Homo sapiens |
Purification (Comment) | Organism |
---|---|
499fold: recombinant UCE expressed in CHO-K1 cells, 1392fold: recombinant UCE expressed in Sf9 insect cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
L-cell | mouse WT 1-38 L-cells expressing human wild-type UCE | Homo sapiens | - |
LoVo cell | derived from a lymph node metastasis of a human colon adenocarcinoma, CCL-229, extremely low UCE activity due to a lack of the activating endoprotease furin, addition of furin restores UCE activity | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.2 | - |
recombinant UCE expressed in CHO-K1 cells | Homo sapiens |
4.75 | - |
recombinant UCE expressed in Sf9 insect cells | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-O-(N-acetyl-alpha-D-glucosaminyl)phosphono-D-mannopyranose + H2O | - |
Homo sapiens | N-acetyl-D-glucosamine + 6-O-phosphono-D-mannopyranose | - |
? | |
glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O | removes a covering N-acetylglucosamine from the mannose 6-phosphate recognition marker on lysosomal acid hydrolases | Homo sapiens | N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose | - |
? | |
methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose + H2O | - |
Homo sapiens | N-acetyl-D-glucosamine + methyl alpha-D-mannose 6-phosphate | - |
? | |
UDP-N-acetyl-D-glucosamine + H2O | - |
Homo sapiens | UDP + N-acetyl-D-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | composed of two disulfide-linked homodimers | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
UCE | uncovering enzyme | Homo sapiens |