Literature summary for 3.1.4.41 extracted from

de Santi Ferrara, G.I.; Fernandes-Pedrosa, M.D.; Junqueira-de-Azevedo, I.D.; Goncalves-de-Andrade, R.M.; Portaro, F.C.; Manzoni-de-Almeida, D.; Murakami, M.T.; Arni, R.K.; van den Berg, C.W.; Ho, P.L.; Tambourgi, D.V.
SMase II, a new sphingomyelinase D from Loxosceles laeta venom gland: Molecular cloning, expression, function and structural analysis (2009), Toxicon, 53, 743-753.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Loxosceles laeta

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	contains Mg2+	Loxosceles laeta

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	His6-tagged protein Smase II, SDS-PAGE	Loxosceles laeta

Organism

Organism	UniProt	Comment	Textmining
Loxosceles laeta	Q8I912	-	-
Loxosceles laeta	Q8I914	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-chelating Sepharose column chromatography	Loxosceles laeta

Source Tissue

Source Tissue	Comment	Organism	Textmining
venom	-	Loxosceles laeta	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sphingomyelin + H2O	-	Loxosceles laeta	ceramide phosphate + choline	-	?

Synonyms

Synonyms	Comment	Organism
SMase D	-	Loxosceles laeta
Smase D 1	-	Loxosceles laeta
SMase I	recombinant enzyme	Loxosceles laeta
SMase II	recombinant enzyme	Loxosceles laeta
sphingomyelinase D	-	Loxosceles laeta
sphingomyelinase D 1	-	Loxosceles laeta

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.004	-	sphingomyelin	crude enzyme, in HEPES-buffered saline at 37°C	Loxosceles laeta
0.0065	-	sphingomyelin	recombinant enzyme SMase I, in HEPES-buffered saline at 37°C	Loxosceles laeta
0.0175	-	sphingomyelin	recombinant enzyme SMase II, in HEPES-buffered saline at 37°C	Loxosceles laeta

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Release 2023.1 (January 2023)