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Literature summary for 3.1.4.4 extracted from
- Purification, biochemical characterization, and cloning of phospholipase D from Streptomyces racemochromogenes strain 10-3 (2010), Protein J., 29, 598-608.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pld103, DNA and amino acid sequence determination and analysis, sequence comparisons | Streptomyces racemochromogenes |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Streptomyces racemochromogenes |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, SDS-PAGE | Streptomyces racemochromogenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces racemochromogenes | E0D7I2 | - |
- |
| Streptomyces racemochromogenes 10-3 | E0D7I2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 17.4fold by ammonium sulfate fractionation, and cation and anion exchange chromatography | Streptomyces racemochromogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 374.3 | - |
purified PLD103, pH 7.5, 37°C | Streptomyces racemochromogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | egg yolk lecithin | Streptomyces racemochromogenes | choline + phosphatidate | - |
? | |
| phosphatidylcholine + H2O | egg yolk lecithin | Streptomyces racemochromogenes 10-3 | choline + phosphatidate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE | Streptomyces racemochromogenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PLD103 | - |
Streptomyces racemochromogenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Streptomyces racemochromogenes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 60 | over 80% of the activity remaining after 10 min within the temperature range | Streptomyces racemochromogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
PLD103 exerts the highest hydrolytic activity at a slightly alkaline pH | Streptomyces racemochromogenes |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | purified enzyme, over 70% activity remaining after 4 h | Streptomyces racemochromogenes |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Streptomyces racemochromogenes | isoelectric focusing | - |
8 |
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