Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Arf1 protein | activates PLD, acts synergistically with CtBP1/BARS in activation of PLD, dependent on GTPgammaS or GTP but not on GDP, overview | Rattus norvegicus | |
CtBP1/BARS | a short splice variant of the CtBP1 gene and a physiological activator of PLD1 required in agonist-induced macropinocytosis. Stimulation of cells by serum or EGF results in the association of CtBP1/BARS with PLD1, which is specifically blocked by 1-butanol. CtBP1/BARS acts synergistically with ARF1 protein in activation of PLD, dependent on GTPgammaS or GTP but not on GDP, overview | Rattus norvegicus | |
additional information | activation mechanism of PLD in macropinocytosis, overview | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
co-expression of the respective wild-type PLD isoforms or lipase-negative (LN)-PLD1 or LN-PLD2inactive mutants in PLD1 or PLD2 KO mutant cells, respectively | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the inhibition of EGF-induced macropinocytosis caused either by PLD1 or PLD2 knockdown is rescued by the co-expression of the respective wild-type PLD isoforms, whereas the expression of a lipase inactive mutant, lipase-negative (LN)-PLD1 or LN-PLD2, does not restore macropinocytotic activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-butanol | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | PLD performs two different reactions: a hydrolytic reaction and a transphosphatidylation reaction, 1-butanol serves as acceptor in the transphosphatidylation reaction, while 2-butanol does not. PLD-catalysed PtdOH formation may be necessary for EGF-induced macropinocytosis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
isozymes PLD1 and PLD2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PLD performs two different reactions: a hydrolytic reaction and a transphosphatidylation reaction, 1-butanol serves as acceptor in the transphosphatidylation reaction, while 2-butanol does not. PLD-catalysed PtdOH formation may be necessary for EGF-induced macropinocytosis | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phospholipase D1 | - |
Rattus norvegicus |
PLD1 | - |
Rattus norvegicus |
PLD2 | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
physiological function | vesicular trafficking such as macropinocytosis is a dynamic process that requires coordinated interactions between specialized proteins and lipids that involves PLD and its physiological activator, the fission protein CtBP1/BARS in agonist-induced macropinocytosis, overview. Molecular mechanisms of lipid remodelling regulation during macropinocytosis. PLD-catalysed PtdOH formation may be necessary for EGF-induced macropinocytosis | Rattus norvegicus |