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Literature summary for 3.1.4.4 extracted from

  • Kokusho, Y.; Kato, S.; Machida, H.; Iwasaki, S.
    Purification and properties of phospholipase D from Actinomadura sp.strain no.362 (1987), Agric. Biol. Chem., 51, 2515-2524.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
cholic acid
-
Actinomadura sp.
ethyl ether stimulates Actinomadura sp.
sodium deoxycholate
-
Actinomadura sp.
Triton X-100 substrate-dependent activation Actinomadura sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag2+ in the presence of Triton X-100 Actinomadura sp.
Al3+ stimulates in the absence of Triton X-100 Actinomadura sp.
Cetylpyridinium chloride
-
Actinomadura sp.
cetyltrimethylammonium bromide
-
Actinomadura sp.
Fe2+ stimulates in the absence of Triton X-100 Actinomadura sp.
Fe3+ stimulates in the absence of Triton X-100 Actinomadura sp.
Hg2+ in the presence of Triton X-100 Actinomadura sp.
Mn2+ stimulates in the absence of Triton X-100 Actinomadura sp.
SDS
-
Actinomadura sp.
Sn2+ stimulates in the absence of Triton X-100 Actinomadura sp.
Triton X-100 substrate-dependent inhibition Actinomadura sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
phosphatidylcholine in the presence of Triton X-100 Actinomadura sp.
1.07
-
lysophosphatidylcholine in the presence of Triton X-100 Actinomadura sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Actinomadura sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ stimulates in the absence of Triton X-100 Actinomadura sp.
Ca2+
-
Actinomadura sp.
Fe2+ stimulates in the absence of Triton X-100 Actinomadura sp.
Fe3+ stimulates in the absence of Triton X-100 Actinomadura sp.
Mn2+ stimulates in the absence of Triton X-100 Actinomadura sp.
Sn2+ stimulates in the absence of Triton X-100 Actinomadura sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000 60000 gel filtration, SDS-PAGE Actinomadura sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipid + alcohol Actinomadura sp. transphosphaditylation phospholipid + alcohol
-
?
phospholipid + H2O Actinomadura sp. phosphoric ester hydrolysis phosphatidic acid + alcohol
-
?

Organism

Organism UniProt Comment Textmining
Actinomadura sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Actinomadura sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
Actinomadura sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
688
-
-
Actinomadura sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lysophosphatidylcholine + H2O
-
Actinomadura sp. monoacylglycerophosphate + choline
-
?
phosphatidylcholine + ethanolamine
-
Actinomadura sp. phosphatidylethanolamine + choline
-
?
phosphatidylcholine + H2O
-
Actinomadura sp. 1,2-diacylglycerophosphate + choline
-
?
phosphatidylethanolamine + H2O
-
Actinomadura sp. 1,2-diacylglycerophosphate + ethanolamine
-
?
phospholipid + alcohol transphosphaditylation Actinomadura sp. phospholipid + alcohol
-
?
phospholipid + H2O phosphoric ester hydrolysis Actinomadura sp. phosphatidic acid + alcohol
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
in the presence of Triton X-100 Actinomadura sp.
60 70 in the absence of Triton X-100 Actinomadura sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
at pH 7.2 for 30 min Actinomadura sp.
30
-
irreversible loss of activity at higher temperature Actinomadura sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7 Triton X-100 dependent Actinomadura sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 2 h at 25°C Actinomadura sp.