Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cholic acid | - |
Actinomadura sp. | |
ethyl ether | stimulates | Actinomadura sp. | |
sodium deoxycholate | - |
Actinomadura sp. | |
Triton X-100 | substrate-dependent activation | Actinomadura sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag2+ | in the presence of Triton X-100 | Actinomadura sp. | |
Al3+ | stimulates in the absence of Triton X-100 | Actinomadura sp. | |
Cetylpyridinium chloride | - |
Actinomadura sp. | |
cetyltrimethylammonium bromide | - |
Actinomadura sp. | |
Fe2+ | stimulates in the absence of Triton X-100 | Actinomadura sp. | |
Fe3+ | stimulates in the absence of Triton X-100 | Actinomadura sp. | |
Hg2+ | in the presence of Triton X-100 | Actinomadura sp. | |
Mn2+ | stimulates in the absence of Triton X-100 | Actinomadura sp. | |
SDS | - |
Actinomadura sp. | |
Sn2+ | stimulates in the absence of Triton X-100 | Actinomadura sp. | |
Triton X-100 | substrate-dependent inhibition | Actinomadura sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
phosphatidylcholine | in the presence of Triton X-100 | Actinomadura sp. | |
1.07 | - |
lysophosphatidylcholine | in the presence of Triton X-100 | Actinomadura sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Actinomadura sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Al3+ | stimulates in the absence of Triton X-100 | Actinomadura sp. | |
Ca2+ | - |
Actinomadura sp. | |
Fe2+ | stimulates in the absence of Triton X-100 | Actinomadura sp. | |
Fe3+ | stimulates in the absence of Triton X-100 | Actinomadura sp. | |
Mn2+ | stimulates in the absence of Triton X-100 | Actinomadura sp. | |
Sn2+ | stimulates in the absence of Triton X-100 | Actinomadura sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | 60000 | gel filtration, SDS-PAGE | Actinomadura sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phospholipid + alcohol | Actinomadura sp. | transphosphaditylation | phospholipid + alcohol | - |
? | |
phospholipid + H2O | Actinomadura sp. | phosphoric ester hydrolysis | phosphatidic acid + alcohol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinomadura sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | Actinomadura sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Actinomadura sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
688 | - |
- |
Actinomadura sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lysophosphatidylcholine + H2O | - |
Actinomadura sp. | monoacylglycerophosphate + choline | - |
? | |
phosphatidylcholine + ethanolamine | - |
Actinomadura sp. | phosphatidylethanolamine + choline | - |
? | |
phosphatidylcholine + H2O | - |
Actinomadura sp. | 1,2-diacylglycerophosphate + choline | - |
? | |
phosphatidylethanolamine + H2O | - |
Actinomadura sp. | 1,2-diacylglycerophosphate + ethanolamine | - |
? | |
phospholipid + alcohol | transphosphaditylation | Actinomadura sp. | phospholipid + alcohol | - |
? | |
phospholipid + H2O | phosphoric ester hydrolysis | Actinomadura sp. | phosphatidic acid + alcohol | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
in the presence of Triton X-100 | Actinomadura sp. |
60 | 70 | in the absence of Triton X-100 | Actinomadura sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
at pH 7.2 for 30 min | Actinomadura sp. |
30 | - |
irreversible loss of activity at higher temperature | Actinomadura sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | Triton X-100 dependent | Actinomadura sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | 2 h at 25°C | Actinomadura sp. |