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Literature summary for 3.1.4.4 extracted from

  • Witt, W.; Yelenosky, G.; Mayer, R.T.
    Purification of phospholipase D from citrus callus tissue (1987), Arch. Biochem. Biophys., 259, 164-170.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
SDS maximal activation at 1 mM Citrus sinensis

General Stability

General Stability Organism
purified enzyme is unstable Citrus sinensis

Inhibitors

Inhibitors Comment Organism Structure
EGTA 96% inhibition at 5 mM Citrus sinensis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 20-100 mM required for maximal activity in plants Citrus sinensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90500 94200 gel filtration, SDS-PAGE Citrus sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipid + alcohol Citrus sinensis transphosphaditylation phospholipid + alcohol
-
?
phospholipid + H2O Citrus sinensis phosphoric ester hydrolysis phosphatidic acid + alcohol
-
?

Organism

Organism UniProt Comment Textmining
Citrus sinensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Citrus sinensis

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon callus tissue Citrus sinensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.66
-
-
Citrus sinensis

Storage Stability

Storage Stability Organism
-15°C, Tris, 2.5 mM DTT, 18 days, 95% loss of activity Citrus sinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + ethanol
-
Citrus sinensis phosphatidylethanol + choline
-
?
phosphatidylcholine + ethanolamine
-
Citrus sinensis phosphatidylethanolamine + choline
-
?
phosphatidylcholine + H2O
-
Citrus sinensis 1,2-diacylglycerophosphate + choline
-
?
phospholipid + alcohol transphosphaditylation Citrus sinensis phospholipid + alcohol
-
?
phospholipid + H2O phosphoric ester hydrolysis Citrus sinensis phosphatidic acid + alcohol
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Citrus sinensis

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5
-
Citrus sinensis