Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | heat shock induces Mg2+-dependent neutral SMase activity in ZE cells as early as 1 h after exposure and reaches a peak of activity 2 h after heat shock | Danio rerio |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysE cells | Danio rerio |
Protein Variants | Comment | Organism |
---|---|---|
H272A | mutant shows reduced activity | Danio rerio |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | in the case of HEK293 transfectants, the zebrafish SMase 1 is also colocalized in both the Golgi apparatus and the endoplasmic reticulum | Danio rerio | 5783 | - |
Golgi apparatus | SMase 1 is also localized in the Golgi apparatus | Danio rerio | 5794 | - |
microsome | in zebrafish embryonic cells, the endogenous SMase enzyme is localized in the microsomal fraction | Danio rerio | - |
- |
additional information | no activity in cytosol | Danio rerio | - |
- |
nucleus | low activity in nuclear fractions | Danio rerio | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Danio rerio |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46900 | - |
recombinant enzyme, putative molecular weight calculated from amino acid sequence and on SDS-PAGE | Danio rerio |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Danio rerio | neutral SMase 1 participates in an inducible ceramide-mediating, proapoptotic signaling pathway that operates in heat-induced apoptosis in zebrafish embryonic cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | Q4LEU0 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap HP column chromatography and Sephacryl S-100 gel filtration | Danio rerio |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Danio rerio | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000000025 | - |
recombinant enzyme, using (1-O-octadecyl-9,10-)lyso-platelet-activating factor as substrate, in 100 mM Tris-HCl, pH 7.5, 5 mM dithiothreitol, 5 mM MgCl2, at 37°C | Danio rerio |
0.00000045 | - |
recombinant enzyme, using sphingomyelin as substrate | Danio rerio |
0.0000264 | - |
wild type enzyme, using C6-7-nitro-2-1,3-benzoxadiazol-4-yl-sphingomyelin as substrate, in 100 mM Tris-HCl, pH 7.5, 5 mM dithiothreitol, 5 mM MgCl2, at 37°C | Danio rerio |
0.0000427 | - |
wild type enzyme after heat shock for 1 h at 37°C, using C6-7-nitro-2-1,3-benzoxadiazol-4-yl-sphingomyelin as substrate, in 100 mM Tris-HCl, pH 7.5, 5 mM dithiothreitol, 5 mM MgCl2, at 37°C | Danio rerio |
0.0000542 | - |
wild type enzyme after heat shock for 1 h at 38°C, using C6-7-nitro-2-1,3-benzoxadiazol-4-yl-sphingomyelin as substrate, in 100 mM Tris-HCl, pH 7.5, 5 mM dithiothreitol, 5 mM MgCl2, at 37°C | Danio rerio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-octadecyl-2-lyso-platelet-activating factor + H2O | very little activity | Danio rerio | 1-O-octadecyl-sn-glycerol + phosphocholine | - |
? | |
C6-7-nitro-2-1,3-benzoxadiazol-4-yl-sphingomyelin + H2O | - |
Danio rerio | ? | - |
? | |
choline-methyl-sphingomyelin + H2O | high activity | Danio rerio | ? | - |
? | |
additional information | neutral SMase 1 participates in an inducible ceramide-mediating, proapoptotic signaling pathway that operates in heat-induced apoptosis in zebrafish embryonic cells | Danio rerio | ? | - |
? | |
additional information | the enzyme has no activity against choline-methyl-phosphatidylcholine | Danio rerio | ? | - |
? | |
sphingomyelin + H2O | - |
Danio rerio | N-acylsphingosine + choline phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neutral SMase 1 | - |
Danio rerio |
neutral sphingomyelinase 1 | neutral SMase 1 acts as a mediator of stress-induced apoptosis | Danio rerio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
recombinant neutral SMase 1 hydrolyzes choline-methyl-sphingomyelin optimally at pH 7.5 in the presence of an Mg2+ ion | Danio rerio |