Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Detergents | - |
Rattus norvegicus | |
Triton X-100 | activation at very low concentrations | Rattus norvegicus |
General Stability | Organism |
---|---|
glycerol, stabilization | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Chloroquine | diphosphate salt, 50% inhibition at 5 mM | Rattus norvegicus | |
dithiothreitol | - |
Rattus norvegicus | |
phosphate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
2n-hexadecanoylamino-4-nitrophenylphosphorylcholine | - |
Rattus norvegicus | |
0.079 | - |
sphingomyelin | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Rattus norvegicus | 5764 | - |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
1 * 45600 + 1 * 44500 + 1 * 40000, SDS-PAGE | Rattus norvegicus |
44500 | - |
1 * 45600 + 1 * 44500 + 1 * 40000, SDS-PAGE | Rattus norvegicus |
45600 | - |
1 * 45600 + 1 * 44500 + 1 * 40000, SDS-PAGE | Rattus norvegicus |
220000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Rattus norvegicus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
5200fold | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
different assay conditions, substrates etc. | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-n-hexadecanoylamino-4-nitrophenylphosphorylcholine + H2O | - |
Rattus norvegicus | 2-n-hexadecanoylamino-4-nitrophenol + choline phosphate | - |
? | |
bis(4-methylumbelliferyl)-phosphate + H2O | poor substrate | Rattus norvegicus | ? | - |
? | |
bis-p-nitrophenyl phosphate + H2O | poor substrate | Rattus norvegicus | ? | - |
? | |
hexadecanoyl-p-nitrophenylphosphorylcholine + H2O | - |
Rattus norvegicus | hexadecanoyl-p-nitrophenol + choline phosphate | - |
? | |
sphingomyelin + H2O | - |
Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 45600 + 1 * 44500 + 1 * 40000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
Chloroquine | diphosphate salt | Rattus norvegicus |