Crystallization (Comment) | Organism |
---|---|
unliganded enzyme, sitting drop vapor diffusion method, using 25% (w/v) PEG3350, 200 mM (NH4)2SO4, and 100 mM Bis-Tris (pH 6.5). Enzyme with AMP and phosphate bound in the active site, sitting drop vapor diffusion method, using 20% (w/v) PEG 2000 MME and 100 mM Bis-Tris (pH 6.0) | Chromobacterium violaceum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
5'-phosphoadenylyl-(3',5')-adenosine | at pH 7.5 and 30°C | Chromobacterium violaceum | |
0.0052 | - |
2'-deoxy-guanosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
0.0054 | - |
N6-methyl adenosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
0.0088 | - |
uridine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
0.0093 | - |
inosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
0.0102 | - |
2'-deoxy-adenosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
0.014 | - |
cytosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
0.0155 | - |
adenosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
0.0173 | - |
guanosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
0.022 | - |
2'-deoxy-inosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
0.026 | - |
2'-deoxy-cytosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
0.029 | - |
2'-deoxy-uridine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM used in assay conditions | Chromobacterium violaceum | |
Mn2+ | the enzyme has three Mn2+ ions in the active site | Chromobacterium violaceum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromobacterium violaceum | Q7NXD4 | - |
- |
Chromobacterium violaceum ATCC 12472 | Q7NXD4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxy-adenosine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | 2'-deoxy-adenosine 5'-phosphate + phosphate | - |
? | |
2'-deoxy-adenosine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum ATCC 12472 | 2'-deoxy-adenosine 5'-phosphate + phosphate | - |
? | |
2'-deoxy-cytosine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | 2'-deoxy-cytosine 5'-phosphate + phosphate | - |
? | |
2'-deoxy-cytosine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum ATCC 12472 | 2'-deoxy-cytosine 5'-phosphate + phosphate | - |
? | |
2'-deoxy-guanosine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | 2'-deoxy-guanosine 5'-phosphate + phosphate | - |
? | |
2'-deoxy-inosine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | 2'-deoxy-inosine 5'-phosphate + phosphate | - |
? | |
2'-deoxy-uridine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | 2'-deoxy-uridine 5'-phosphate + phosphate | - |
? | |
5'-phosphoadenylyl-(3',5')-adenosine + H2O | lowest activity | Chromobacterium violaceum | ? | - |
? | |
5'-phosphoguanylyl-(3',5')-adenosine + H2O | - |
Chromobacterium violaceum | ? | - |
? | |
adenosine 3',5'-bisphosphate + H2O | second highest activity | Chromobacterium violaceum | adenosine 5'-phosphate + phosphate | - |
? | |
adenosine 3',5'-bisphosphate + H2O | second highest activity | Chromobacterium violaceum ATCC 12472 | adenosine 5'-phosphate + phosphate | - |
? | |
cytosine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | cytosine 5'-phosphate + phosphate | - |
? | |
guanosine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | guanosine 5'-phosphate + phosphate | - |
? | |
guanosine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum ATCC 12472 | guanosine 5'-phosphate + phosphate | - |
? | |
inosine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | inosine 5'-phosphate + phosphate | - |
? | |
N6-methyl adenosine 3',5'-bisphosphate + H2O | highest activity | Chromobacterium violaceum | N6-methyl adenosine 5'-phosphate + phosphate | - |
? | |
uridine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | uridine 5'-phosphate + phosphate | - |
? | |
uridine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum ATCC 12472 | uridine 5'-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | x-ray crystallography | Chromobacterium violaceum |
Synonyms | Comment | Organism |
---|---|---|
3',5'-nucleotide bisphosphate phosphatase | - |
Chromobacterium violaceum |
Cv1693 | - |
Chromobacterium violaceum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
5'-phosphoadenylyl-(3',5')-adenosine | at pH 7.5 and 30°C | Chromobacterium violaceum | |
3.3 | - |
inosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
4.2 | - |
2'-deoxy-guanosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
4.3 | - |
2'-deoxy-inosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
5.2 | - |
2'-deoxy-uridine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
5.4 | - |
uridine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
7.1 | - |
2'-deoxy-adenosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
8.6 | - |
cytosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
9.4 | - |
2'-deoxy-cytosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
11.6 | - |
N6-methyl adenosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
12.3 | - |
guanosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
22 | - |
adenosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32 | - |
5'-phosphoadenylyl-(3',5')-adenosine | at pH 7.5 and 30°C | Chromobacterium violaceum | |
180 | - |
2'-deoxy-uridine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
190 | - |
2'-deoxy-inosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
360 | - |
2'-deoxy-cytosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
360 | - |
inosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
610 | - |
uridine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
620 | - |
cytosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
700 | - |
2'-deoxy-adenosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
710 | - |
guanosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
790 | - |
2'-deoxy-guanosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
1400 | - |
adenosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum | |
2100 | - |
N6-methyl adenosine 3',5'-bisphosphate | at pH 7.5 and 30°C | Chromobacterium violaceum |