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nucleoside-3',5'-bisphosphate 3'-phosphohydrolase
The enzyme, characterized from the bacterium Chromobacterium violaceum, has similar catalytic efficiencies with all the bases. The enzyme has similar activity with ribonucleoside and 2'-deoxyribonucleoside 3',5'-bisphosphates, but shows no activity with nucleoside 2',5'-bisphosphates (cf. EC 3.1.3.7, 3'(2'),5'-bisphosphate nucleotidase).
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2'-deoxy-adenosine 3',5'-bisphosphate + H2O
2'-deoxy-adenosine 5'-phosphate + phosphate
Substrates: -
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2'-deoxy-cytidine 3',5'-bisphosphate + H2O
2'-deoxy-cytidine 5'-phosphate + phosphate
Substrates: -
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2'-deoxy-guanosine 3',5'-bisphosphate + H2O
2'-deoxy-guanosine 5'-phosphate + phosphate
Substrates: -
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2'-deoxy-inosine 3',5'-bisphosphate + H2O
2'-deoxy-inosine 5'-phosphate + phosphate
Substrates: -
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2'-deoxy-uridine 3',5'-bisphosphate + H2O
2'-deoxy-uridine 5'-phosphate + phosphate
Substrates: -
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5'-phosphoadenylyl-(3',5')-adenosine + H2O
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Substrates: lowest activity
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5'-phosphoguanylyl-(3',5')-adenosine + H2O
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Substrates: -
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adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
Substrates: second highest activity
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cytidine 3',5'-bisphosphate + H2O
cytidine 5'-phosphate + phosphate
Substrates: -
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guanosine 3',5'-bisphosphate + H2O
guanosine 5'-phosphate + phosphate
Substrates: -
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inosine 3',5'-bisphosphate + H2O
inosine 5'-phosphate + phosphate
Substrates: -
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N6-methyl adenosine 3',5'-bisphosphate + H2O
N6-methyl adenosine 5'-phosphate + phosphate
Substrates: highest activity
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uridine 3',5'-bisphosphate + H2O
uridine 5'-phosphate + phosphate
Substrates: -
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0.0102
2'-deoxy-adenosine 3',5'-bisphosphate
at pH 7.5 and 30°C
0.026
2'-deoxy-cytosine 3',5'-bisphosphate
at pH 7.5 and 30°C
0.0052
2'-deoxy-guanosine 3',5'-bisphosphate
at pH 7.5 and 30°C
0.022
2'-deoxy-inosine 3',5'-bisphosphate
at pH 7.5 and 30°C
0.029
2'-deoxy-uridine 3',5'-bisphosphate
at pH 7.5 and 30°C
0.0018
5'-phosphoadenylyl-(3',5')-adenosine
at pH 7.5 and 30°C
0.0155
adenosine 3',5'-bisphosphate
at pH 7.5 and 30°C
0.014
cytosine 3',5'-bisphosphate
at pH 7.5 and 30°C
0.0173
guanosine 3',5'-bisphosphate
at pH 7.5 and 30°C
0.0093
inosine 3',5'-bisphosphate
at pH 7.5 and 30°C
0.0054
N6-methyl adenosine 3',5'-bisphosphate
at pH 7.5 and 30°C
0.0088
uridine 3',5'-bisphosphate
at pH 7.5 and 30°C
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7.1
2'-deoxy-adenosine 3',5'-bisphosphate
at pH 7.5 and 30°C
9.4
2'-deoxy-cytosine 3',5'-bisphosphate
at pH 7.5 and 30°C
4.2
2'-deoxy-guanosine 3',5'-bisphosphate
at pH 7.5 and 30°C
4.3
2'-deoxy-inosine 3',5'-bisphosphate
at pH 7.5 and 30°C
5.2
2'-deoxy-uridine 3',5'-bisphosphate
at pH 7.5 and 30°C
0.05
5'-phosphoadenylyl-(3',5')-adenosine
at pH 7.5 and 30°C
22
adenosine 3',5'-bisphosphate
at pH 7.5 and 30°C
8.6
cytosine 3',5'-bisphosphate
at pH 7.5 and 30°C
12.3
guanosine 3',5'-bisphosphate
at pH 7.5 and 30°C
3.3
inosine 3',5'-bisphosphate
at pH 7.5 and 30°C
11.6
N6-methyl adenosine 3',5'-bisphosphate
at pH 7.5 and 30°C
5.4
uridine 3',5'-bisphosphate
at pH 7.5 and 30°C
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700
2'-deoxy-adenosine 3',5'-bisphosphate
at pH 7.5 and 30°C
360
2'-deoxy-cytosine 3',5'-bisphosphate
at pH 7.5 and 30°C
790
2'-deoxy-guanosine 3',5'-bisphosphate
at pH 7.5 and 30°C
190
2'-deoxy-inosine 3',5'-bisphosphate
at pH 7.5 and 30°C
180
2'-deoxy-uridine 3',5'-bisphosphate
at pH 7.5 and 30°C
32
5'-phosphoadenylyl-(3',5')-adenosine
at pH 7.5 and 30°C
1400
adenosine 3',5'-bisphosphate
at pH 7.5 and 30°C
620
cytosine 3',5'-bisphosphate
at pH 7.5 and 30°C
710
guanosine 3',5'-bisphosphate
at pH 7.5 and 30°C
360
inosine 3',5'-bisphosphate
at pH 7.5 and 30°C
2100
N6-methyl adenosine 3',5'-bisphosphate
at pH 7.5 and 30°C
610
uridine 3',5'-bisphosphate
at pH 7.5 and 30°C
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Cummings, J.A.; Vetting, M.; Ghodge, S.V.; Xu, C.; Hillerich, B.; Seidel, R.D.; Almo, S.C.; Raushel, F.M.
Prospecting for unannotated enzymes: discovery of a 3',5'-nucleotide bisphosphate phosphatase within the amidohydrolase superfamily
Biochemistry
53
591-600
2014
Chromobacterium violaceum (Q7NXD4), Chromobacterium violaceum, Chromobacterium violaceum ATCC 12472 (Q7NXD4)
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