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Literature summary for 3.1.3.7 extracted from

  • Zhang, J.Y.; Zou, J.; Bao, Q.; Chen, W.L.; Wang, L.; Yang, H.; Zhang, C.C.
    A lithium-sensitive and sodium-tolerant 3-phosphoadenosine-5-phosphatase encoded by halA from the cyanobacterium Arthrospira platensis is closely related to its counterparts from yeasts and plants (2006), Appl. Environ. Microbiol., 72, 245-251.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Limnospira platensis

Inhibitors

Inhibitors Comment Organism Structure
Li+
-
 Limnospira platensis
Na+
-
 Limnospira platensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.61
-
 adenosine 3'-phosphate 5'-phosphosulfate in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30°C Limnospira platensis
1.03
-
 adenosine 3',5'-bisphosphate in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30°C Limnospira platensis
41.5
-
 5'-ADP in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30°C Limnospira platensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the activity increases over increasing concentrations of Mg2+ to reach its optimal values in the presence of 2 to 2.5 mM Mg2+ Limnospira platensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41800
-
 SDS-PAGE Limnospira platensis

Organism

Organism UniProt Comment Textmining
Limnospira platensis Q3LS17 formerly known as Arthrospira platensis
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity chromatography Limnospira platensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-ADP + H2O
-
 Limnospira platensis ?
-
 ?
adenosine 3',5'-bisphosphate + H2O 100% activity Limnospira platensis adenosine 5'-phosphate + phosphate
-
 ?
adenosine 3'-phosphate 5'-phosphosulfate + H2O 42% activity compared to adenosine 3',5'bisphosphate Limnospira platensis adenosine 5'-phosphosulfate + phosphate
-
 ?
inositol-1,4-bisphosphate + H2O 5.6% activity compared to adenosine 3',5'bisphosphate Limnospira platensis inositol 4-phosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
3-phosphoadenosine-5-phosphatase
-
 Limnospira platensis
HalA
-
 Limnospira platensis
PAPase
-
 Limnospira platensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Limnospira platensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
10
-
 remained 60% of maximal activity when the pH of the reaction sample is increased to pH 10 Limnospira platensis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.6
-
 in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30°C Limnospira platensis Li+
600
-
 in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30°C Limnospira platensis Na+