Cloned (Comment) | Organism |
---|---|
MIPP cDNA from Jurkat T-cells is cloned and expressed in Escherichia coli M15 without the ER-targeting domain, full-length sequence | Homo sapiens |
MIPP cDNA from liver and kidney is cloned and expressed in Escherichia coli M15, full-length sequence | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
H370A | mutant with less than 5% of activity of wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
D-myo-Inositol 1,3,4,5-tetrakisphosphate | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | ER-based protein, N-terminal ER-targeting domain | Rattus norvegicus | 5783 | - |
endoplasmic reticulum | ER-based protein, N-terminal ER-targeting domain | Homo sapiens | 5783 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mg2+-independent | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
x * 51000, recombinant MIPP expressed in Escherichia coli M15, SDS-PAGE and Western blot analysis | Homo sapiens |
53400 | - |
x * 53400, recombinant MIPP expressed in Escherichia coli M15, amino acid sequence analysis | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | MIPP mRNA is upregulated during chondrocyte hypertrophy, MIPP may aid bone mineralization and salvage the inositol moiety prior to apoptosis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UNW1 | - |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant MIPP expressed in Escherichia coli M15 | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
chondrocyte | MIPP expression pattern in developing chondrocytes | Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
kidney | 2.5 kb mRNA expression | Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
liver | 2.5 kb mRNA expression | Homo sapiens | - |
additional information | not expressed in peripheral blood leukocytes | Homo sapiens | - |
placenta | 2.5 kb mRNA expression | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O | - |
Rattus norvegicus | D-myo-inositol 1,4,5-trisphosphate + phosphate | - |
? | |
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O | - |
Homo sapiens | D-myo-inositol 1,4,5-trisphosphate + phosphate | - |
? | |
inositol hexakisphosphate + H2O | - |
Rattus norvegicus | inositol pentakisphosphate + phosphate | - |
? | |
additional information | dephosphorylates inositol signaling molecules InsP5 and InsP6 | Rattus norvegicus | ? | - |
? | |
additional information | catalytic requirement for the C-terminus and His-370 of MIPP | Homo sapiens | ? | - |
? | |
additional information | MIPP mRNA is upregulated during chondrocyte hypertrophy, MIPP may aid bone mineralization and salvage the inositol moiety prior to apoptosis | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53400, recombinant MIPP expressed in Escherichia coli M15, amino acid sequence analysis | Homo sapiens |
? | x * 51000, recombinant MIPP expressed in Escherichia coli M15, SDS-PAGE and Western blot analysis | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
More | structural and functional relationship between MIPP and histidine acid phosphatase | Rattus norvegicus |
More | structural and functional relationship between MIPP and histidine acid phosphatase | Homo sapiens |