Information on EC 3.1.3.62 - multiple inositol-polyphosphate phosphatase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
3.1.3.62
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RECOMMENDED NAME
GeneOntology No.
multiple inositol-polyphosphate phosphatase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
myo-inositol hexakisphosphate + H2O = myo-inositol pentakisphosphate (mixed isomers) + phosphate
show the reaction diagram
formerly D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O = D-myo-inositol 1,4,5-trisphosphate + phosphate
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-myo-inositol (1,3,4)-trisphosphate biosynthesis
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-
phytate degradation I
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-
phytate degradation II
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Inositol phosphate metabolism
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-
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SYSTEMATIC NAME
IUBMB Comments
1D-myo-inositol-hexakisphosphate 5-phosphohydrolase
This enzyme exists in two isoforms. It also acts on Ins(1,3,4,5)P4 to yield Ins(1,4,5)P3.
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CAS REGISTRY NUMBER
COMMENTARY hide
116958-30-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
avian
-
-
Manually annotated by BRENDA team
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A0FHA7, A0FHA8, A0FHA9
SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
male Japanese white rabbits, 4 weeks old
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3-bisphosphoglycerate + H2O
2-phosphoglycerate + phosphate
show the reaction diagram
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
?
D-myo-inositol 1,2,3,4,5,6-hexakisphosphate + H2O
D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
show the reaction diagram
-
-
-
-
?
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + phosphate
show the reaction diagram
inositol 1,3,4,5,6-pentakisphosphate + H2O
inositol 1,4,5,6-tetrakisphosphate + phosphate
show the reaction diagram
inositol 1,3,4,6-tetrakisphosphate + H2O
?
show the reaction diagram
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very poor substrate
-
-
?
inositol 3,4,5,6-tetrakisphosphate + H2O
?
show the reaction diagram
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about 10% of activity with inositol 1,3,4,5-tetrakisphosphate
-
-
?
inositol hexakisphosphate + H2O
inositol pentakisphosphate + phosphate
show the reaction diagram
myo-inositol hexakisphosphate + H2O
myo-inositol pentakisphosphate + phosphate
show the reaction diagram
-
-
-
-
?
scyllo-inositol hexakisphosphate + H2O
scyllo-inositol pentakisphosphate + phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
D-myo-inositol 1,4,5-trisphosphate + phosphate
show the reaction diagram
-
enzyme may be regulated by the intracellular concentrations of inositol tris- and tetrakisphosphates
-
?
inositol 1,3,4,5,6-pentakisphosphate + H2O
inositol 1,4,5,6-tetrakisphosphate + phosphate
show the reaction diagram
-
inositol pentakisphosphate and inositol hexakisphosphate are likely to be the preferred substrates in vivo
-
?
inositol hexakisphosphate + H2O
inositol pentakisphosphate + phosphate
show the reaction diagram
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inositol pentakisphosphate and inositol hexakisphosphate are likely to be the preferred substrates in vivo
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?
additional information
?
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KCl
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activation
additional information
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ammonium phosphate buffer
ATP
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weak
CTP
-
more effective inhibitor than GTP
Endogenous heat-stable inhibitor
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GTP
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marked inhibition
Inositol 1,3,4,5,6-pentakisphosphate
inositol 1,3,4-trisphosphate
Inositol 1,4,5-trisphosphate
inositol 3,4,5,6-tetrakisphosphate
Inositol hexakisphosphate
UTP
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more effective inhibitor than GTP
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ammonium phosphate buffer
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1 to 10 mM activates, higher concentrations are inhibitory
hemoglobin
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from turkey, activates by removing the inhibitors inositol 1,3,4,5,6-pentakisphosphate and inositol hexakisphosphate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.61
2,3-bisphosphoglycerate
-
recombinant enzyme, at 37°C
0.00013 - 0.008
D-myo-Inositol 1,3,4,5-tetrakisphosphate
0.00004
Inositol 1,3,4,5,6-pentakisphosphate
-
pH 7.4, 37°C
0.0000003 - 0.334
Inositol hexakisphosphate
0.06 - 0.14
myo-inositol hexakisphosphate
1.74
scyllo-inositol hexakisphosphate
-
-
additional information
additional information
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kinetic data of the cytosolic enzyme
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000025 - 0.00006
Inositol 1,3,4,5,6-pentakisphosphate
0.00175
inositol 1,3,4-trisphosphate
-
pH 7.4, 37°C
0.002
Inositol 1,4,5-trisphosphate
-
pH 7.4, 37°C
0.0005
inositol 3,4,5,6-tetrakisphosphate
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pH 7.4, 37°C
0.0000005 - 0.000003
Inositol hexakisphosphate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.009
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using 2,3-bisphosphoglycerate as substrate, at 37°C
0.7
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specific activity of recombinant avian MINPP
4.29
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pH 7.4, liver enzyme
4.66
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pH 7.4, 37°C, membrane-bound enzyme
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
assay at
5 - 9
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plateau within the range, inositol 1,3,4,5,6-pentakisphosphate or inositol hexakisphosphate as substrate, in absence of Mg2+
6.1
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inositol 1,3,4,5-tetrakisphosphate as substrate, in absence of Mg2+
8
assay at; assay at
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
assay at
additional information
-
assay at room temperature
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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chondrocyte progenitor cell line, developmentally specific changes in MINPP expression
Manually annotated by BRENDA team
mRNA expression
Manually annotated by BRENDA team
mRNA expression
Manually annotated by BRENDA team
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lymphoma cell line
Manually annotated by BRENDA team
mRNA expression; mRNA expression
Manually annotated by BRENDA team
mRNA expression
Manually annotated by BRENDA team
mRNA expression; mRNA expression
Manually annotated by BRENDA team
2.5 kb mRNA expression
Manually annotated by BRENDA team
mRNA expression; mRNA expression
Manually annotated by BRENDA team
mRNA expression
Manually annotated by BRENDA team
mRNA expression
Manually annotated by BRENDA team
mRNA expression; mRNA expression
Manually annotated by BRENDA team
mRNA expression
Manually annotated by BRENDA team
additional information
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47000
-
1 * 47000, liver enzyme, doublet, SDS-PAGE
51000
x * 51000, recombinant MIPP expressed in Escherichia coli M15, SDS-PAGE and Western blot analysis
53400
x * 53400, recombinant MIPP expressed in Escherichia coli M15, amino acid sequence analysis
53800
SDS-PAGE; SDS-PAGE
54500
x * 54500, inclusive the putative signal sequence, amino acid sequence analysis
55100
x * 55100, inclusive the putative signal sequence, amino acid sequence analysis
64000
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SDS-PAGE
66000
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particulate enzyme, gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.4
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 37
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 20% v/v glycerol, 1 mg/ml bovine serum albumin, up to 12 months, stable
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-80°C, at least 3 months
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-80°C, several months, stable
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0°C, 10 mM Tris-HCl, pH 7.4, 100 mM KCl, 1 mM dithiothreitol, up to 1 month, stable
4°C, within weeks, loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
22400fold from liver
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Ni-Sepharose column chromatography
partial
particulate enzyme: 38374fold, soluble enzyme: partial
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purification is carried out on a Ni-sepharose column
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recombinant (MMU)Minpp1 fusion protein expressed in Escherichia coli JM109
recombinant MIPP expressed in Escherichia coli M15
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cDNA encoding residues 19-445 of avian MINPP or residues 31-483 of human MINPP lacking the N-terminal ER targeting sequences and the C-terminal ER retention signals are amplified by PCR and cloned into pGEM Tvector and transformed into Escherichia coli
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cloned in Escherichia coli; expressed in Escherichia coli
expressed in Escherichia coli
expressed in Pichia pastoris
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full-length Minpp cDNA is cloned and stably overexpressed in murine ATDC-5 cells at 2fold higher levels than in wild-type ATDC-5 cells, cellular effects of overexpression
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His-tag fusion, expression in Pichia pastoris
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located on chromosome 10q23, genomic organization
MINPP gene encoding multiple inositol polyphosphate phosphatase
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MIPP cDNA from Jurkat T-cells is cloned and expressed in Escherichia coli M15 without the ER-targeting domain, full-length sequence
MIPP cDNA from liver and kidney is cloned and expressed in Escherichia coli M15, full-length sequence
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N-terminal truncated cytosolic MIPP is expressed in the beta-cell line HIT M2.2.2, expression increases the basal Ca2+-concentration to a more normal level
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sequenced and expressed in Escherichia coli JM109, located on chromosome 19, genomic organization
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Q78A
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Km: 0.06 (myo-inositol hexakisphosphate). Q78A mutant shows differences in its specificity profile compared to wild-type enzyme: relative to inositol hexakisphosphate, the activity towards other organic phosphates are as follows: p-nitrophenyl phosphate: 7%, ATP: 12%, ribose 1-phosphate: 3%
T27G
-
Km: 0.092 (myo-inositol hexakisphosphate)
H370A
mutant with less than 5% of activity of wild-type enzyme
H89A
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catalytically compromised mutant of MIPP1 that shows only 1% of the 2,3-diphosphoglycerate phosphatase activity of the wild type enzyme
additional information
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Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-affinity chromatography
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
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recombinant avian MINPP (0.7 micromol/mg protein/min) is the most active phytase found to date in any animal cell
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LINKS TO OTHER DATABASES (specific for EC-Number 3.1.3.62)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)