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Literature summary for 3.1.3.62 extracted from

  • Nogimori, K.; Hughes, P.J.; Glennon, M.C.; Hodgson, M.E.; Putney, J.W.; Shears, S.B.
    Purification of an inositol (1,3,4,5)-tetrakisphosphate 3-phosphatase activity from rat liver and the evaluation of its substrate specificity (1991), J. Biol. Chem., 266, 16499-16506.
    View publication on PubMed
Search for more literature for 3.1.3.62

Activating Compound

Activating Compound Comment Organism Structure
hemoglobin from turkey, activates by removing the inhibitors inositol 1,3,4,5,6-pentakisphosphate and inositol hexakisphosphate Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Inositol 1,3,4,5,6-pentakisphosphate competitive; strong, kinetics; turkey hemoglobin reverses Rattus norvegicus
inositol 1,3,4-trisphosphate
-
 Rattus norvegicus
Inositol 1,4,5-trisphosphate product inhibition kinetics Rattus norvegicus
inositol 3,4,5,6-tetrakisphosphate
-
 Rattus norvegicus
Inositol hexakisphosphate competitive, turkey hemoglobin reverses; more effective than inositol pentakisphosphate; strong, kinetics Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000003
-
 Inositol hexakisphosphate pH 7.4, 37°C Rattus norvegicus
0.00004
-
 Inositol 1,3,4,5,6-pentakisphosphate pH 7.4, 37°C Rattus norvegicus
0.00013
-
 D-myo-Inositol 1,3,4,5-tetrakisphosphate pH 7.4, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
particle-bound predominantly in particulate fraction Rattus norvegicus
-
-
soluble
-
 Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2.5fold activation at 5-10 mM, no activation at 1 mM and physiologically relevant concentrations Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 particulate enzyme, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
inositol 1,3,4,5,6-pentakisphosphate + H2O Rattus norvegicus inositol pentakisphosphate and inositol hexakisphosphate are likely to be the preferred substrates in vivo inositol 1,4,5,6-tetrakisphosphate + phosphate
-
 ?
inositol hexakisphosphate + H2O Rattus norvegicus inositol pentakisphosphate and inositol hexakisphosphate are likely to be the preferred substrates in vivo inositol pentakisphosphate + phosphate
-
 ?
additional information Rattus norvegicus enzyme is relatively unimportant to inositol 1,3,4,5-tetrakisphosphate metabolism in vivo ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 male sprague-dawley rats
-

Purification (Commentary)

Purification (Comment) Organism
particulate enzyme: 38374fold, soluble enzyme: partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.66
-
 pH 7.4, 37°C, membrane-bound enzyme Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, at least 3 months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
-
 Rattus norvegicus D-myo-inositol 1,4,5-trisphosphate + phosphate
-
 ?
inositol 1,3,4,5,6-pentakisphosphate + H2O
-
 Rattus norvegicus inositol 1,4,5,6-tetrakisphosphate + phosphate
-
 ?
inositol 1,3,4,5,6-pentakisphosphate + H2O inositol pentakisphosphate and inositol hexakisphosphate are likely to be the preferred substrates in vivo Rattus norvegicus inositol 1,4,5,6-tetrakisphosphate + phosphate
-
 ?
inositol 1,3,4,6-tetrakisphosphate + H2O very poor substrate Rattus norvegicus ?
-
 ?
inositol 3,4,5,6-tetrakisphosphate + H2O about 10% of activity with inositol 1,3,4,5-tetrakisphosphate Rattus norvegicus ?
-
 ?
inositol hexakisphosphate + H2O
-
 Rattus norvegicus inositol pentakisphosphate + phosphate at least 4 isomers: D- and/or L-inositol 1,2,4,5,6-pentakisphosphate, D-inositol 1,3,4,5,6-pentakisphosphate, D-inositol 1,2,3,4,6-pentakisphosphate and D- and/or L-inositol 1,2,3,4,5-pentakisphosphate ?
inositol hexakisphosphate + H2O inositol pentakisphosphate and inositol hexakisphosphate are likely to be the preferred substrates in vivo Rattus norvegicus inositol pentakisphosphate + phosphate
-
 ?
additional information not: inositol 1,3,4-trisphosphate, inositol 1,3-bisphosphate, inositol 3,4-bisphosphate, 4-nitrophenyl phosphate Rattus norvegicus ?
-
 ?
additional information enzyme is relatively unimportant to inositol 1,3,4,5-tetrakisphosphate metabolism in vivo Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 66000, particulate enzyme, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 9 plateau within the range, inositol 1,3,4,5,6-pentakisphosphate or inositol hexakisphosphate as substrate, in absence of Mg2+ Rattus norvegicus
6.1
-
 inositol 1,3,4,5-tetrakisphosphate as substrate, in absence of Mg2+ Rattus norvegicus
7.4
-
 inositol hexakisphosphate as substrate, plus 1 mM Mg2+ Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000005
-
 Inositol hexakisphosphate pH 7.4, 37°C Rattus norvegicus
0.000025
-
 Inositol 1,3,4,5,6-pentakisphosphate pH 7.4, 37°C Rattus norvegicus