Activating Compound | Comment | Organism | Structure |
---|---|---|---|
hemoglobin | from turkey, activates by removing the inhibitors inositol 1,3,4,5,6-pentakisphosphate and inositol hexakisphosphate | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Inositol 1,3,4,5,6-pentakisphosphate | competitive; strong, kinetics; turkey hemoglobin reverses | Rattus norvegicus | |
inositol 1,3,4-trisphosphate | - |
Rattus norvegicus | |
Inositol 1,4,5-trisphosphate | product inhibition kinetics | Rattus norvegicus | |
inositol 3,4,5,6-tetrakisphosphate | - |
Rattus norvegicus | |
Inositol hexakisphosphate | competitive, turkey hemoglobin reverses; more effective than inositol pentakisphosphate; strong, kinetics | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000003 | - |
Inositol hexakisphosphate | pH 7.4, 37°C | Rattus norvegicus | |
0.00004 | - |
Inositol 1,3,4,5,6-pentakisphosphate | pH 7.4, 37°C | Rattus norvegicus | |
0.00013 | - |
D-myo-Inositol 1,3,4,5-tetrakisphosphate | pH 7.4, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
particle-bound | predominantly in particulate fraction | Rattus norvegicus | - |
- |
soluble | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 2.5fold activation at 5-10 mM, no activation at 1 mM and physiologically relevant concentrations | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
particulate enzyme, gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
inositol 1,3,4,5,6-pentakisphosphate + H2O | Rattus norvegicus | inositol pentakisphosphate and inositol hexakisphosphate are likely to be the preferred substrates in vivo | inositol 1,4,5,6-tetrakisphosphate + phosphate | - |
? | |
inositol hexakisphosphate + H2O | Rattus norvegicus | inositol pentakisphosphate and inositol hexakisphosphate are likely to be the preferred substrates in vivo | inositol pentakisphosphate + phosphate | - |
? | |
additional information | Rattus norvegicus | enzyme is relatively unimportant to inositol 1,3,4,5-tetrakisphosphate metabolism in vivo | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male sprague-dawley rats | - |
Purification (Comment) | Organism |
---|---|
particulate enzyme: 38374fold, soluble enzyme: partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.66 | - |
pH 7.4, 37°C, membrane-bound enzyme | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, at least 3 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O | - |
Rattus norvegicus | D-myo-inositol 1,4,5-trisphosphate + phosphate | - |
? | |
inositol 1,3,4,5,6-pentakisphosphate + H2O | - |
Rattus norvegicus | inositol 1,4,5,6-tetrakisphosphate + phosphate | - |
? | |
inositol 1,3,4,5,6-pentakisphosphate + H2O | inositol pentakisphosphate and inositol hexakisphosphate are likely to be the preferred substrates in vivo | Rattus norvegicus | inositol 1,4,5,6-tetrakisphosphate + phosphate | - |
? | |
inositol 1,3,4,6-tetrakisphosphate + H2O | very poor substrate | Rattus norvegicus | ? | - |
? | |
inositol 3,4,5,6-tetrakisphosphate + H2O | about 10% of activity with inositol 1,3,4,5-tetrakisphosphate | Rattus norvegicus | ? | - |
? | |
inositol hexakisphosphate + H2O | - |
Rattus norvegicus | inositol pentakisphosphate + phosphate | at least 4 isomers: D- and/or L-inositol 1,2,4,5,6-pentakisphosphate, D-inositol 1,3,4,5,6-pentakisphosphate, D-inositol 1,2,3,4,6-pentakisphosphate and D- and/or L-inositol 1,2,3,4,5-pentakisphosphate | ? | |
inositol hexakisphosphate + H2O | inositol pentakisphosphate and inositol hexakisphosphate are likely to be the preferred substrates in vivo | Rattus norvegicus | inositol pentakisphosphate + phosphate | - |
? | |
additional information | not: inositol 1,3,4-trisphosphate, inositol 1,3-bisphosphate, inositol 3,4-bisphosphate, 4-nitrophenyl phosphate | Rattus norvegicus | ? | - |
? | |
additional information | enzyme is relatively unimportant to inositol 1,3,4,5-tetrakisphosphate metabolism in vivo | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 66000, particulate enzyme, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | plateau within the range, inositol 1,3,4,5,6-pentakisphosphate or inositol hexakisphosphate as substrate, in absence of Mg2+ | Rattus norvegicus |
6.1 | - |
inositol 1,3,4,5-tetrakisphosphate as substrate, in absence of Mg2+ | Rattus norvegicus |
7.4 | - |
inositol hexakisphosphate as substrate, plus 1 mM Mg2+ | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000005 | - |
Inositol hexakisphosphate | pH 7.4, 37°C | Rattus norvegicus | |
0.000025 | - |
Inositol 1,3,4,5,6-pentakisphosphate | pH 7.4, 37°C | Rattus norvegicus |