Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90100 | - |
SDS-PAGE, maltose binding protein MBP-Type I ins(1,4,5)P3 5-phosphatase fusion protein | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification by a dual tag strategy: cDNA encoding human Type I Ins(1,4,5)P3 5-phosphatase is subcloned into a modified pMAL expression vector. This plasmid produces a recombinant protein in fusion with affinity tags located at its N-terminus, consisting in a maltose binding protein (MPB) and an octa-histidine stretch. The construction is transformed into Escherichia coli BL21 (DE3) expression strain. Yield: 18 mg of pure and stable enzyme starting from a 2 L | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.2 | - |
measured in the presence of 0.05 mM of labeled Ins(1,4,5)P3 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1D-myo-inositol 1,4,5-trisphosphate + H2O | - |
Homo sapiens | 1D-myo-inositol 1,4-disphosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
inositol(1,4,5)P3 5-phosphatase | - |
Homo sapiens |
Ins(1,4,5)P3 5-phosphatase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |