Literature summary for 3.1.3.56 extracted from

Wohlkoenig, A.; Senechal, M.; Dewitte, F.; Backers, K.; Erneux, C.; Villeret, V.
Expression and purification in high yield of a functionally active recombinant human Type I inositol(1,4,5)P3 5-phosphatase (2007), Protein Expr. Purif., 55, 69-74.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
90100	-	SDS-PAGE, maltose binding protein MBP-Type I ins(1,4,5)P3 5-phosphatase fusion protein	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purification by a dual tag strategy: cDNA encoding human Type I Ins(1,4,5)P3 5-phosphatase is subcloned into a modified pMAL expression vector. This plasmid produces a recombinant protein in fusion with affinity tags located at its N-terminus, consisting in a maltose binding protein (MPB) and an octa-histidine stretch. The construction is transformed into Escherichia coli BL21 (DE3) expression strain. Yield: 18 mg of pure and stable enzyme starting from a 2 L	Homo sapiens

Purification (Comment)

Organism

purification by a dual tag strategy: cDNA encoding human Type I Ins(1,4,5)P3 5-phosphatase is subcloned into a modified pMAL expression vector. This plasmid produces a recombinant protein in fusion with affinity tags located at its N-terminus, consisting in a maltose binding protein (MPB) and an octa-histidine stretch. The construction is transformed into Escherichia coli BL21 (DE3) expression strain. Yield: 18 mg of pure and stable enzyme starting from a 2 L

Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.2	-	measured in the presence of 0.05 mM of labeled Ins(1,4,5)P3	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1D-myo-inositol 1,4,5-trisphosphate + H2O	-	Homo sapiens	1D-myo-inositol 1,4-disphosphate + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
inositol(1,4,5)P3 5-phosphatase	-	Homo sapiens
Ins(1,4,5)P3 5-phosphatase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
7	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens