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Literature summary for 3.1.3.56 extracted from

  • Tsujishita, Y.; Guo, S.; Stolz, L.E.; York, J.D.; Hurley, J.H.
    Specificity determinants in phosphoinositide dephosphorylation: crystal structure of an archetypal inositol polyphosphate 5-phosphatase (2001), Cell, 105, 379-389.
    View publication on PubMed
Search for more literature for 3.1.3.56

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae inp51,52,53, lacking inositol 1,4,5-trisphosphate 5-phosphatase activity, rescue of cell viability in cloned cells, His-tag fusion protein expressed in Escherichia coli BL21 Schizosaccharomyces pombe

Crystallization (Commentary)

Crystallization (Comment) Organism
in dialysis bags for 20 h Schizosaccharomyces pombe

Protein Variants

Protein Variants Comment Organism
D838G no activity Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 D-myo-inositol 1,4,5-trisphosphate
-
 Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O Schizosaccharomyces pombe
-
 D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
D-myo-inositol 1,4,5-trisphosphate + H2O Schizosaccharomyces pombe regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tag fusion protein expressed in Escherichia coli Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(3-sn-phosphatidyl)-L-myo-inositol 4,5-bisphosphate + H2O
-
 Schizosaccharomyces pombe phosphatidylinositol 4-phosphate + phosphate
-
 ?
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
-
 Schizosaccharomyces pombe D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
D-myo-inositol 1,4,5,6-tetrakisphosphate + H2O poor substrate Schizosaccharomyces pombe D-myo-inositol 1,4,6-trisphosphate + phosphate
-
 ?
D-myo-inositol 1,4,5-trisphosphate + H2O
-
 Schizosaccharomyces pombe D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
D-myo-inositol 1,4,5-trisphosphate + H2O regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate Schizosaccharomyces pombe D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
phosphatidyl-L-myo-inositol 3,4,5-trisphosphate + H2O
-
 Schizosaccharomyces pombe phosphatidylinositol 3,4-bisphosphate + phosphate
-
 ?