Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Tequatrovirus T4 |
Protein Variants | Comment | Organism |
---|---|---|
D167N | the mutant converts a RNA 2',3'-cyclic phosphate end to RNA 3'-phosphate | Tequatrovirus T4 |
S211A | the mutant transiently accumulates phosphor-RNA during 2',3'-cyclic phosphate removal | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phospho-5'-hydroxy-RNA + H2O | Tequatrovirus T4 | - |
? | - |
? | |
a RNA 2',3'-cyclic phosphate end + H2O | Tequatrovirus T4 | the enzyme also has RNA 2'-phosphatase activity | a RNA 3'-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-agarose column chromatography | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phospho-5'-hydroxy-RNA + H2O | - |
Tequatrovirus T4 | ? | - |
? | |
a RNA 2',3'-cyclic phosphate end + H2O | the enzyme also has RNA 2'-phosphatase activity | Tequatrovirus T4 | a RNA 3'-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PNKP | - |
Tequatrovirus T4 |
polynucleotide kinase-phosphatase | - |
Tequatrovirus T4 |